核酸合成中的计算机程序：利用具有数据存储、检索和分析能力的固相化学技术进行合成策略开发。

Computer programs in nucleic acid synthesis: synthetic strategy development using solid-phase chemical techniques with data storage, retrieval and analysis capabilities.

作者信息

Lombardi S, Seidell H, Pulford S, Dutton W, Parekh S

出版信息

Nucleic Acids Res. 1984 Mar 12;12(5):2581-91. doi: 10.1093/nar/12.5.2581.

DOI:10.1093/nar/12.5.2581

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318687/

Abstract

A computer program has been designed to aid development of synthetic strategies for oligonucleotides produced by solid-phase chemical techniques. The program reduces the time required to develop a strategy and a data file from hours to minutes. The program contains inventories, provides cost analyses, and generates and stores other associated data. The program searches an inventory of sequences for that sequence to avoid duplicate synthesis. If the sequence is not in the inventory the program devises a synthetic strategy, calculates the amounts of reagents and labor costs necessary to complete the synthetic oligonucleotide. The program also deducts the reagents from inventory files. Physical data is also calculated. A file is generated in a sequence inventory for storage of the data as well as other data that will be generated during the purification processes. All variable parameters can be easily edited. The programs were designed to provide a cross-referencing feature for data analysis and can use several parameters as a constant.

摘要

已设计出一种计算机程序，以辅助制定通过固相化学技术生产寡核苷酸的合成策略。该程序将制定策略和生成数据文件所需的时间从数小时缩短至数分钟。该程序包含库存清单，提供成本分析，并生成和存储其他相关数据。该程序在序列清单中搜索该序列，以避免重复合成。如果该序列不在清单中，程序会设计一种合成策略，计算完成合成寡核苷酸所需的试剂用量和人工成本。该程序还会从库存文件中扣除试剂。还会计算物理数据。会在序列清单中生成一个文件，用于存储数据以及纯化过程中生成的其他数据。所有可变参数都可轻松编辑。这些程序旨在为数据分析提供交叉引用功能，并可将多个参数用作常量。

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本文引用的文献

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Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.寡脱氧核糖核苷酸的快速合成。IV. 通过磷酸三酯中间体改进寡脱氧核糖核苷酸的固相合成

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5

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Nucleic Acids Res. 1981 Sep 25;9(18):4611-26. doi: 10.1093/nar/9.18.4611.

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Total synthesis of a human leukocyte interferon gene.人白细胞干扰素基因的全合成

Nature. 1981 Aug 20;292(5825):756-62. doi: 10.1038/292756a0.

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Studies on polynucleotides. 93. A further study of the synthesis of deoxyribopolynucleotides using preformed oligonucleotide blocks.

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CVI. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast. Synthesis of two nonanucleotides and a heptanucleotide corresponding to nucleotide sequences 22 to 30, 41 to 49 and 28 to 34.

J Mol Biol. 1972 Dec 28;72(2):289-307. doi: 10.1016/0022-2836(72)90149-0.

10

Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.芳基磺酰四唑，新型偶联试剂以及脱氧核糖寡核苷酸合成三酯法的进一步改进。

Nucleic Acids Res. 1977 Feb;4(2):353-71. doi: 10.1093/nar/4.2.353.

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