Harley E H
Nucleic Acids Res. 1986 Jan 10;14(1):467-77. doi: 10.1093/nar/14.1.467.
A program is described to perform general DNA sequence analysis on the Hewlett-Packard Model 86/87 microcomputer operating on 128 K of RAM. The following analytical procedures can be performed: 1. display of the sequence, in whole or part, or its complement; 2. search for specified sequences e.g. restriction sites, and in the case of the latter give fragment sizes; 3. perform a comprehensive search for all known restriction enzyme sites; 4. map sites graphically; 5. perform editing functions; 6. base frequency analysis; 7. search for repeated sequences; 8. search for open reading frames or translate into the amino acid sequence and analyse for basic and acidic amino acids, hydrophobicity, and codon usage. Two sequences, or parts thereof, can be merged in various orientations to mimic recombination strategies, or can be compared for homologies. The program is written in HP BASIC and is designed principally as a tool for the laboratory investigator manipulating a defined set of vectors and recombinant DNA constructs.
本文描述了一个程序，用于在配备128K随机存取存储器的惠普86/87型微型计算机上进行常规DNA序列分析。可以执行以下分析程序：1. 显示全部或部分序列及其互补序列；2. 搜索特定序列，例如限制酶切位点，对于后者给出片段大小；3. 全面搜索所有已知的限制酶位点；4. 以图形方式绘制位点图谱；5. 执行编辑功能；6. 碱基频率分析；7. 搜索重复序列；8. 搜索开放阅读框或翻译成氨基酸序列，并分析碱性和酸性氨基酸、疏水性及密码子使用情况。两个序列或其部分可以以各种方向合并以模拟重组策略，或者可以比较同源性。该程序用惠普BASIC语言编写，主要设计为实验室研究人员操作特定载体和重组DNA构建体的工具。
