China-Canada Joint Lab of Food Nutrition and Health (Beijing), Beijing Technology and Business University, Beijing 100048, China.
College of Basic Science, Tianjin Agricultural University, Tianjin 300392, China.
Molecules. 2021 Aug 4;26(16):4713. doi: 10.3390/molecules26164713.
, the food-borne bacteria present in dairy products, ready-to-eat food and environmental sources, has been reported with antibiotic resistance, raising concerns about food microbial safety. The antimicrobial resistance of requires the development of new strategies. Light- and photosensitizer-based antimicrobial photodynamic inactivation (PDI) is a promising approach to control microbial contamination, whereas there is limited information regarding the effectiveness of PDI on biofilm control. In this study, PDI mediated by natural bioactive compound (curcumin) associated with LED was evaluated for its potential to prevent and disrupt biofilms. Biofilms were treated with curcumin (50, 100, 200 µM) and LED fluence (4.32 J/cm, 8.64 J/cm, 17.28 J/cm). Control groups included samples treated only with curcumin or light, and samples received neither curcumin nor light. The action was examined on biofilm mass, viability, cellular metabolic activity and cytoplasmic membrane integrity. PDI using curcumin associated with LED exhibited significant antibiofilm activities, inducing biofilm prevention and removal, metabolic inactivation, intracellular membrane damage and cell death. Likewise, scanning electronic microscopy observations demonstrated obvious structural injury and morphological alteration of biofilm after PDI application. In conclusion, curcumin is an effective photosensitizer for the photodynamic control of biofilm.
食源性细菌存在于乳制品、即食食品和环境来源中，已被报道具有抗生素耐药性，这引起了人们对食品微生物安全的关注。需要制定新的策略来应对 的抗药性。光和光敏剂为基础的抗菌光动力灭活（PDI）是一种控制微生物污染的有前途的方法，但是关于 PDI 对 生物膜控制的有效性的信息有限。在这项研究中，评估了与 LED 相关的天然生物活性化合物（姜黄素）介导的 PDI 对预防和破坏 的生物膜的潜在作用。用 curcumin（50、100、200 μM）和 LED 剂量（4.32 J/cm、8.64 J/cm、17.28 J/cm）处理生物膜。对照组包括仅用 curcumin 或光处理的样品，以及既未用 curcumin 也未用光处理的样品。检查了对生物膜质量、活力、细胞代谢活性和细胞质膜完整性的作用。与 LED 相关的 curcumin 介导的 PDI 表现出显著的抗生物膜活性，诱导生物膜的预防和去除、代谢失活、细胞内膜损伤和细胞死亡。同样，扫描电子显微镜观察表明，PDI 应用后， 生物膜的结构损伤和形态改变明显。总之，姜黄素是一种有效的光动力控制 生物膜的光敏剂。
