三重态-三重态湮灭上转换基氧传感器克服自发荧光的限制。
Triplet-Triplet Annihilation Upconversion-Based Oxygen Sensors to Overcome the Limitation of Autofluorescence.
机构信息
Chemical and Biological Engineering, Colorado School of Mines, Golden, Colorado 80401, United States.
Quantitative Biosciences and Engineering, Colorado School of Mines, Golden, Colorado 80401, United States.
出版信息
ACS Sens. 2023 Aug 25;8(8):3043-3050. doi: 10.1021/acssensors.3c00548. Epub 2023 Aug 4.
Autofluorescence is one of the many challenges in bioimaging as it can mask the emission from fluorescent probes or markers, a limitation that can be overcome via upconversion. Herein, we have developed a nanosensor that uses triplet-triplet annihilation upconversion to optically report changes in the dissolved oxygen concentration. Using a sensitizer-annihilator dye pairing of platinum(II) octaethylporphyrin and 9,10-diphenylanthracene, we monitored the oxygen consumption (as a proxy for metabolic activity) over time in a biological system─ (brewing yeast). The nanosensor demonstrated good reversibility over multiple cycles and showed good signal and colloidal stability when tested over the course of 7 days, and it was sensitive to dissolved oxygen from 0.00 to 3.17 mg/L O. Additionally, there was no signal overlap between the nanosensor emission and autofluorescence, thus underscoring the utility of upconversion as a facile and economical means of overcoming autofluorescence.
自发荧光是生物成像中的众多挑战之一，因为它可以掩盖荧光探针或标记物的发射，这种限制可以通过上转换来克服。在此，我们开发了一种纳米传感器，该传感器利用三重态-三重态湮灭上转换来光学报告溶解氧浓度的变化。我们使用敏化剂-猝灭剂染料对铂（II）八乙基卟啉和 9,10-二苯基蒽进行配对，以监测一段时间内生物系统（酿酒酵母）中的耗氧量（作为代谢活性的指标）。该纳米传感器在多个循环中表现出良好的可逆性，并且在 7 天的测试过程中表现出良好的信号和胶体稳定性，其对 0.00 至 3.17 mg/L O 的溶解氧具有敏感性。此外，纳米传感器发射与自发荧光之间没有信号重叠，因此凸显了上转换作为一种简便、经济的克服自发荧光的方法的实用性。
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