Laboratory of Natural Nutraceuticals Biotesting, Kemerovo State University, 650043 Kemerovo, Russia.
Department of Bionanotechnology, Kemerovo State University, 650043 Kemerovo, Russia.
Int J Mol Sci. 2023 Jul 25;24(15):11906. doi: 10.3390/ijms241511906.
The antimicrobial properties of baicalin against and several probiotic cultures were evaluated. Baicalin was isolated from a dry plant extract obtained by extraction with water at 70 °C. For isolation, extraction was carried out with n-butanol and purification on a chromatographic column. The antimicrobial potential was assessed by evaluating changes in the optical density of the bacterial suspension during cultivation; additionally, the disk diffusion method was used. During the study, the baicalin concentrations (0.25, 0.5, and 1 mg/mL) and the pH of the medium in the range of 1.5-8.0 were tested. The test objects were: suspensions of , , , , and . It was found that the greater the concentration of the substance in the solution, the greater the delay in the growth of the strain zone. Thus, the highest antimicrobial activity against was observed at pH 1.5-2.0 and a baicalin concentration of 1.00 mg/mL. In relation to probiotic strains, a stimulating effect of baicalin (1.00 mg/mL) on the growth of biomass at pH 1.5-2.0 was observed. The results open up the prospects for the use of baicalin and probiotics for the treatment of diseases caused by .
我们评估了黄芩苷对 和几种益生菌培养物的抗菌性能。黄芩苷是从 70°C 水提取的干燥植物提取物中分离出来的。为了进行分离，采用正丁醇进行提取，并在色谱柱上进行纯化。通过评估细菌悬浮液在培养过程中光密度的变化来评估抗菌潜力；此外，还使用了圆盘扩散法。在研究过程中，测试了黄芩苷浓度（0.25、0.5 和 1 mg/mL）和培养基 pH 值在 1.5-8.0 范围内的变化。测试对象是： 、 、 、 和 悬浮液。结果发现，溶液中物质的浓度越高，菌株区生长的延迟就越大。因此，在 pH 值为 1.5-2.0 且黄芩苷浓度为 1.00 mg/mL 时，观察到对 的抗菌活性最高。关于益生菌株，在 pH 值为 1.5-2.0 时，观察到黄芩苷（1.00 mg/mL）对 生物量生长有刺激作用。这些结果为黄芩苷和益生菌联合用于治疗 引起的疾病开辟了前景。
