Dragonieri Silvano, Quaranta Vitaliano Nicola, Portacci Andrea, Ahroud Madiha, Di Marco Marcin, Ranieri Teresa, Carpagnano Giovanna Elisiana
Department of Respiratory Diseases, University of Bari "Aldo Moro", 70121 Bari, Italy.
Molecules. 2023 Jul 30;28(15):5755. doi: 10.3390/molecules28155755.
Exhaled breath analysis using an e-nose is a groundbreaking tool for exhaled volatile organic compound (VOC) analysis, which has already shown its applicability in several respiratory and systemic diseases. It is still unclear whether food intake can be considered a confounder when analyzing the VOC-profile. We aimed to assess whether an e-nose can discriminate exhaled breath before and after predefined food intake at different time periods. We enrolled 28 healthy non-smoking adults and collected their exhaled breath as follows: (a) before food intake, (b) within 5 min after food consumption, (c) within 1 h after eating, and (d) within 2 h after eating. Exhaled breath was collected by a formerly validated method and analyzed by an e-nose (Cyranose 320). By principal component analysis, significant variations in the exhaled VOC-profile were shown for principal component 1 (capturing 63.4% of total variance) when comparing baseline vs. 5 min and vs. 1 h after food intake (both < 0.05). No significance was shown in the comparison between baseline and 2 h after food intake. Therefore, the exhaled VOC-profile seems to be influenced by very recent food intake. Interestingly, two hours might be sufficient to avoid food induced alterations of exhaled VOC-spectrum when sampling for research protocols.
使用电子鼻进行呼气分析是一种用于呼出挥发性有机化合物（VOC）分析的开创性工具，它已在多种呼吸系统和全身性疾病中显示出适用性。在分析VOC谱时，食物摄入是否可被视为一个混杂因素仍不清楚。我们旨在评估电子鼻能否区分在不同时间段摄入预定义食物前后的呼气。我们招募了28名健康的非吸烟成年人，并按以下方式收集他们的呼气：（a）进食前，（b）进食后5分钟内，（c）进食后1小时内，以及（d）进食后2小时内。呼气通过先前验证的方法收集，并由电子鼻（Cyranose 320）进行分析。通过主成分分析，在比较基线与进食后5分钟以及进食后1小时的主成分1（占总方差的63.4%）时，呼出的VOC谱显示出显著变化（两者均<0.05）。在比较基线与进食后2小时时未显示出显著性。因此，呼出的VOC谱似乎受近期食物摄入的影响。有趣的是，在为研究方案进行采样时，两小时可能足以避免食物引起的呼出VOC谱变化。
