Institute of Public Health and Clinical Nutrition, University of Eastern Finland, P.O. Box 1627, FI-70211 Kuopio, Finland.
SIB Labs, University of Eastern Finland, P.O. Box 1627, FI-70211 Kuopio, Finland.
Molecules. 2021 May 2;26(9):2667. doi: 10.3390/molecules26092667.
Exhaled breath is a potential noninvasive matrix to give new information about metabolic effects of diets. In this pilot study, non-targeted analysis of exhaled breath volatile organic compounds (VOCs) was made by comprehensive two-dimensional gas chromatography-mass spectrometry (GCxGC-MS) to explore compounds relating to whole grain (WG) diets. Nine healthy subjects participated in the dietary intervention with parallel crossover design, consisting of two high-fiber diets containing whole grain rye bread (WGR) or whole grain wheat bread (WGW) and 1-week control diets with refined wheat bread (WW) before both diet periods. Large interindividual differences were detected in the VOC composition. About 260 VOCs were detected from exhaled breath samples, in which 40 of the compounds were present in more than half of the samples. Various derivatives of benzoic acid and phenolic compounds, as well as some furanones existed in exhaled breath samples only after the WG diets, making them interesting compounds to study further.
呼气是一种潜在的非侵入性基质，可以提供有关饮食代谢影响的新信息。在这项初步研究中，通过全面二维气相色谱-质谱联用（GCxGC-MS）对呼出的挥发性有机化合物（VOC）进行非靶向分析，以探索与全谷物（WG）饮食有关的化合物。9 名健康受试者参与了平行交叉设计的饮食干预，包括两种高纤维饮食，分别含有全麦黑麦面包（WGR）或全麦小麦面包（WGW），以及在这两种饮食期之前各 1 周的精制小麦面包（WW）对照饮食。在 VOC 组成方面，检测到了个体间存在较大差异。从呼出的呼吸样本中检测到约 260 种 VOC，其中 40 种化合物存在于一半以上的样本中。只有在 WG 饮食后，呼出的呼吸样本中才存在各种苯甲酸和酚类化合物的衍生物，以及一些呋喃酮，这使得它们成为进一步研究的有趣化合物。
