Department of Orthopedic Surgery, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China; Hunan Engineering Research Center of Biomedical Metal and Ceramic Implants, Changsha, China.
Department of Orthopaedics, The Third Xiangya Hospital Central South University, 138 Tongzipo Road, Changsha, Hunan China..
Colloids Surf B Biointerfaces. 2023 Oct;230:113506. doi: 10.1016/j.colsurfb.2023.113506. Epub 2023 Aug 7.
Electrospinning technology, as a novel approach, has been extensively applied in the field of tissue engineering. Nanofiber membranes prepared by electrospinning can effectively mimic the structure and function of natural bone matrix, providing an ideal scaffold for attachment, proliferation, and differentiation of bone cells while inducing osteogenic differentiation and new bone formation. However, it lacks bioactivities such as osteoinduction, angiogenesis and the ability to promote nerve regeneration. In the presence of complex critical bone defects, a single component electrospun membrane often fails to suffice for bone repair needs. Based on this, we prepared a biofunctionalized membrane loaded with Tantalum(Ta)/Whitlockite(WH) nanoparticles (poly-ε-caprolactone (PCL)/Ta/WH) in order to promote high-quality bone defect repair through neurovascular coupling effect. According to the results of in vitro and in vivo experiments, the early Mg release of WH can effectively increase the local nerve and vascular density, and synergize with Tantalum nanoparticles (TaNPs) to create a rich nerve-vascular microenvironment. This allows the PCL/Ta/WH membrane to repair bone defects in multiple dimensions and achieve high-quality repair of bone tissue, providing new solutions for the treatment of critical bone defects in clinical.
静电纺丝技术作为一种新型方法，已广泛应用于组织工程领域。静电纺丝制备的纳米纤维膜可以有效模拟天然骨基质的结构和功能，为骨细胞的附着、增殖和分化提供理想的支架，同时诱导成骨分化和新骨形成。然而，它缺乏成骨诱导、血管生成和促进神经再生等生物活性。在存在复杂的临界骨缺损的情况下，单一成分的静电纺丝膜通常无法满足骨修复的需求。基于此，我们制备了一种负载钽（Ta）/羟磷灰石（WH）纳米颗粒的生物功能化膜（聚己内酯（PCL）/Ta/WH），以通过神经血管耦联效应促进高质量的骨缺损修复。根据体外和体内实验结果，WH 的早期 Mg 释放可以有效增加局部神经和血管密度，并与钽纳米颗粒（TaNPs）协同作用，形成丰富的神经血管微环境。这使得 PCL/Ta/WH 膜能够从多个维度修复骨缺损，实现骨组织的高质量修复，为临床治疗临界骨缺损提供了新的解决方案。
