Department of Biochemistry & Molecular Biology, Sealy Center for Structural Biology & Molecular Biophysics, University of Texas Medical Branch, Galveston, TX 77555, USA.
Department of Biochemistry & Molecular Biology, Sealy Center for Structural Biology & Molecular Biophysics, University of Texas Medical Branch, Galveston, TX 77555, USA.
Curr Opin Struct Biol. 2023 Oct;82:102680. doi: 10.1016/j.sbi.2023.102680. Epub 2023 Aug 11.
Biomolecular electrostatics has been a subject of computational investigations based on 3D structures. This situation is changing because emerging experimental tools allow us to quantitatively investigate biomolecular electrostatics without any use of structure information. Now, electrostatic potentials around biomolecules can directly be measured for many residues simultaneously by nuclear magnetic resonance (NMR) spectroscopy. This NMR method can be used to study electrostatic aspects of various processes, including macromolecular association and liquid-liquid phase separation. Applications to structurally flexible biomolecules such as intrinsically disordered proteins are particularly useful. The new tools also facilitate examination of theoretical models and methods for biomolecular electrostatics.
生物分子静电学一直是基于 3D 结构的计算研究课题。这种情况正在发生变化，因为新兴的实验工具使我们能够在不使用任何结构信息的情况下定量研究生物分子静电。现在，通过核磁共振（NMR）光谱可以直接同时测量许多残基周围的分子静电势。这种 NMR 方法可用于研究包括大分子缔合和液-液相分离在内的各种过程中的静电方面。它在结构灵活的生物分子（如天然无序蛋白）中的应用尤其有用。这些新工具也有助于检验生物分子静电的理论模型和方法。
