Nouioura Ghizlane, Lafdil Fatima Zahra, Kandsi Fahd, Mohammad Salamatullah Ahmad, Tiji Salima, Soulo Najoua, Giesy John P, Aboul-Soud Mourad A M, Lyoussi Badiaa, Derwich Elhoussine
Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health and Quality of Life (SNAMOPEQ), Faculty of Sciences Dhar El Mehraz, Sidi Mohamed Ben Abdellah University, Fez 30 000, Morocco.
Laboratory of Bio-resources, Biotechnology, Faculty of Sciences, Ethnopharmacology and Health, Mohammed the First University, Oujda 60000, Morocco.
ACS Omega. 2024 Jun 17;9(26):28155-28166. doi: 10.1021/acsomega.4c01107. eCollection 2024 Jul 2.
Anemia is a widespread global health concern necessitating effective, accessible, and natural interventions. The potential of medicinal plants to address anemia has garnered significant interest. Among these plants, parsley ( () L.) stands out as an edible and herbal-based option for combating anemia. Aim of the study: This study investigated the potential of (PC-Ext) as an emerging antianemic product, focusing on its physicochemical attributes, antioxidant properties, and mineral profile. Both qualitative and quantitative analyses of the phenolic compounds in were conducted by using high-performance liquid chromatography with a diode array detector (HPLC-DAD). Anemia was induced in rats by intravenous injections of phenylhydrazine, administered at a dose of 40 mg/kg for two consecutive days. The antianemic activity of PC-Ext was assessed at a dose of 500 mg/kg twice daily for 5 weeks by estimating blood parameters, such as serum iron and ferritin. Additionally, the osmotic fragility test measured the capacity of red blood cells to withstand osmotic shock of various concentrations of saline. Aqueous extract of was rich in phytochemical compounds, including syringic acid, quercetin, catechin, gallic acid, and luteolin. The findings demonstrate the effectiveness of in ameliorating phenylhydrazine-induced reductions in red blood cell count (RBCs), hemoglobin (Hb), and hematocrit (HCT) levels. Consequently, PC-Ext exhibits significant activity against phenylhydrazine-induced anemia in rats, as demonstrated by its ability to prevent hemolysis. Iron estimation within PC-Ext further confirms its utility in addressing both iron deficiency and ferritin-deficiency anemia. Therefore, PC exhibits a favorable effect against both types of anemia, iron deficiency, and hemolysis. The results of this study provide robust scientific validation for ethnomedicinal use and the potential utility of , positioning it as a promising source for future pharmaceutical development.
贫血是一个全球性的广泛健康问题，需要有效的、可及的和天然的干预措施。药用植物解决贫血问题的潜力已引起了广泛关注。在这些植物中，欧芹（() L.）作为一种可食用的、基于草药的抗贫血选择脱颖而出。研究目的：本研究调查了欧芹提取物（PC-Ext）作为一种新兴抗贫血产品的潜力，重点关注其理化特性、抗氧化性能和矿物质成分。通过使用配备二极管阵列检测器的高效液相色谱法（HPLC-DAD）对欧芹中的酚类化合物进行了定性和定量分析。通过连续两天以40 mg/kg的剂量静脉注射苯肼诱导大鼠贫血。通过估计血清铁和铁蛋白等血液参数，以500 mg/kg的剂量每日两次评估PC-Ext的抗贫血活性，持续5周。此外，渗透脆性试验测量了红细胞承受不同浓度盐水渗透冲击的能力。欧芹水提取物富含植物化学化合物，包括丁香酸、槲皮素、儿茶素、没食子酸和木犀草素。研究结果表明，欧芹在改善苯肼诱导的红细胞计数（RBC）、血红蛋白（Hb）和血细胞比容（HCT）水平降低方面是有效的。因此，PC-Ext对大鼠苯肼诱导的贫血表现出显著活性，这通过其预防溶血的能力得到证明。PC-Ext中的铁含量进一步证实了其在解决缺铁性贫血和铁蛋白缺乏性贫血方面的效用。因此，欧芹对两种类型的贫血、缺铁和溶血均表现出良好的效果。本研究结果为欧芹的民族药用和潜在效用提供了有力的科学验证，使其成为未来药物开发的一个有前景的来源。
