Naghibi Mehran, Tayefi Nasrabadi Hamid, Soleimani Rad Jafar, Garjani Alireza, Gholami Farashah Mohammad Sadegh, Mohammadnejad Daryoush
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Int J Fertil Steril. 2023 Aug 7;17(4):268-275. doi: 10.22074/ijfs.2022.544368.1235.
In many diabetic patients, spermatogenesis complications are frequent causing infertility problems. This study aimed to demonstrate the effect of Forskolin on male reproductive dysfunction caused by type 2 diabetes.
In this experimental study, type 2 diabetes was induced by a high-fat diet (HFD) for one month and then a low single dose injection (35 mg/kg) of streptozotocin (STZ) in Wistar rats. After 72 hours, rats with more than 200 mg/dl of blood glucose were considered type 2 diabetic rats. Forty rats (200-250 g) were divided into four groups (n=10) including group 1 (G1): rats with normal diet and buffer citrate (STZ solvent) injection, group 2 (G2): control type 2 diabetic rats with HFD and STZ injection, group 3 (G3): type 2 diabetic rats received phosphate buffer saline (PBS) as Forskolin solvent, and group 4 (G4): Forskolin treated diabetic rats (10 mg/kg) for 1 month.
In comparison to control group, in diabetic groups (G2 and G3) some parameters are increased significantly: The blood glucose (P=0.00078), testicular malondialdehyde (MDA) level and body weight (P=0.00009) and Bax gene expression (P=0.00007). Unlike, some parameters are decreased significantly: The serum level of testosterone (P=0.0009), testicular superoxide dismutase (SOD, P=0.00007) and glutathione peroxidase (GPX) levels (P=0.00008), sperm concentration (P=0.00008), motility (P=0.00009), normal morphological sperm (P=0.00008) and gene expression (P=0.00009). However, in Forskolin treated group (G4) the parameters stayed close to control values that was significantly (P=0.00007) higher than in G2 and G3 groups. Therefore, treatment with Forskolin significantly improved these abnormal changes in Forskolin-treated group.
Our study demonstrates that Forskolin is an effective antidiabetic agent, which significantly improves sperm concentration, testosterone levels, and antioxidant activity in diabetic rats.
在许多糖尿病患者中，精子发生并发症频繁出现，导致不孕问题。本研究旨在证明福斯高林对2型糖尿病所致男性生殖功能障碍的影响。
在本实验研究中，通过给Wistar大鼠喂食高脂饮食（HFD）1个月，然后单次低剂量注射（35mg/kg）链脲佐菌素（STZ）诱导2型糖尿病。72小时后，血糖超过200mg/dl的大鼠被视为2型糖尿病大鼠。40只体重200 - 250g的大鼠被分为四组（n = 10），包括第1组（G1）：正常饮食并注射枸橼酸盐缓冲液（STZ溶剂）的大鼠；第2组（G2）：接受HFD和STZ注射的2型糖尿病对照大鼠；第3组（G3）：接受磷酸盐缓冲盐水（PBS）作为福斯高林溶剂的2型糖尿病大鼠；第4组（G4）：用福斯高林（10mg/kg）治疗1个月的糖尿病大鼠。
与对照组相比，糖尿病组（G2和G3）的一些参数显著升高：血糖（P = 0.00078）、睾丸丙二醛（MDA）水平、体重（P = 0.00009）和Bax基因表达（P = 0.00007）。不同的是，一些参数显著降低：血清睾酮水平（P = 0.0009）、睾丸超氧化物歧化酶（SOD，P = 0.00007）和谷胱甘肽过氧化物酶（GPX）水平（P = 0.00008）、精子浓度（P = 0.00008）、活力（P = 0.00009）、正常形态精子（P = 0.00008）和基因表达（P = 0.
