Ali Zarie Arwa, Osman Magdi A, Alshammari Ghedeir M, Hassan Amro B, ElGasim Ahmed Yagoub Abu, Abdo Yahya Mohammed
Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia.
Saudi J Biol Sci. 2023 Sep;30(9):103732. doi: 10.1016/j.sjbs.2023.103732. Epub 2023 Jul 5.
This research aim was to assess the impact of the seed extracts of the date cultivars (Qatara, Barhi, and Ruthana) on rat's liver steatosis, oxidative stress, and inflammation triggered by feeding a high-fat diet (HFD). The experimental design was based on random partitioning into two groups; one that received the standard diet and another that received the HFD diet. The HFD rats were orally administered Lipitor or date seed extracts at 300 or 600 mg/kg/day for 4 weeks. Accordingly, feeding rats HFD significantly increased body and liver weights, hepatic and serum lipid levels, glucose, insulin, HOMA-IR, liver function enzymes, and inflammation markers, and decreased oxidative stress enzymes. Oral administration of Barhi and Ruthana date seed extracts significantly decreased body and liver weights. Serum and liver total cholesterol TC, Triglycerides TGs, and free fatty acids FFAs were also decreased as were AST, ALT, MAD, leptin, and CRP, with a concomitant increase in SOD, GSH, and CAT. Furthermore, similar to Lipitor, oral administration of the extracts reduced inflammation markers such as TNF-α, serum CRP, IL-6, IL-1β, and leptin while increasing IL-10 and adiponectin levels. Histological observation revealed that extract administration improved hepatocyte and parenchymal structures and decreased lipid deposition. In conclusion, both Barhi and Ruthana seed extracts showed strong hepatoprotective, anti-inflammatory, and antioxidant effects against HFD-induced liver steatosis. And date seeds have other beneficial potential for prevention and treatment of various diseases, which can be studied in the future.
本研究旨在评估枣品种（卡塔拉、巴里和鲁塔纳）的种子提取物对高脂饮食（HFD）引发的大鼠肝脏脂肪变性、氧化应激和炎症的影响。实验设计基于随机分为两组；一组接受标准饮食，另一组接受HFD饮食。给HFD大鼠口服立普妥或枣种子提取物，剂量为300或600毫克/千克/天，持续4周。因此，给大鼠喂食HFD显著增加了体重和肝脏重量、肝脏和血清脂质水平、葡萄糖、胰岛素、HOMA-IR、肝功能酶和炎症标志物，并降低了氧化应激酶。口服巴里和鲁塔纳枣种子提取物显著降低了体重和肝脏重量。血清和肝脏总胆固醇TC、甘油三酯TGs和游离脂肪酸FFAs也有所降低，AST、ALT、MAD、瘦素和CRP也降低，同时SOD、GSH和CAT增加。此外，与立普妥相似，口服提取物可降低炎症标志物，如TNF-α、血清CRP、IL-6、IL-1β和瘦素，同时增加IL-10和脂联素水平。组织学观察显示，提取物给药改善了肝细胞和实质结构，并减少了脂质沉积。总之，巴里和鲁塔纳种子提取物对HFD诱导的肝脏脂肪变性均表现出强大的肝脏保护、抗炎和抗氧化作用。枣种子在预防和治疗各种疾病方面还有其他有益潜力，可在未来进行研究。
