Kongkwamcharoen Chonthicha, Itharat Arunporn, Ketjinda Wichan, Lee Hyang-Yeol, Moon Gi-Seong, Davies Neal M
Graduate School on Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand.
Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand.
Res Pharm Sci. 2023 Mar 10;18(3):244-261. doi: 10.4103/1735-5362.371581. eCollection 2023 May-Jun.
L. has long been used in Thai traditional medicine to treat osteoarthritis and inflammation by placing it on painful areas without further formulation design which is suboptimal for therapeutic use. Thus, this research aims to formulate a topical hydrogel patch containing L. extracts (CAE) for anti-inflammatory effects.
The hydrogel patches are made from carrageenan, locust bean gum, with glycerin as a plasticizer and contain CAE formulated by using response surface methodology based on Box-Behnken design for design, determination of the effect of independent factors on the tensile strength, and optimization of the hydrogel patch formulation. release and skin permeation studies using a modified Franz diffusion cell and anti-inflammatory activity were evaluated.
FINDINGS/RESULTS: The optimized CAE hydrogel patch showed a good correlation between predicted and observed tensile strength values and exerted its maximum cumulative lycorine release and permeation at 69.38 ± 2.78% and 48.51 ± 0.45%, respectively which were fit to Higuchi's kinetic model. The release rates were found to decrease with an increase in the polymer proportion of carrageenan and locust bean gum. In addition, the patch exerted potent anti-inflammatory activity with an IC value of 21.36 ± 0.78 μg/mL.
The optimized CAE hydrogel patch application was successfully formulated with excellent mechanical properties, cumulative release, permeation, and anti-inflammatory effects. Thus, it has the potential to be further developed as a herbal application to relieve pain and inflammation. The anti-inflammatory effect of this delivery system should be further investigated.
长期以来，L. 在泰国传统医学中被用于治疗骨关节炎和炎症，方法是将其敷于疼痛部位，未进行进一步的配方设计，这种方式在治疗应用上并不理想。因此，本研究旨在制备一种含有L. 提取物（CAE）的局部水凝胶贴剂，以发挥抗炎作用。
水凝胶贴剂由角叉菜胶、刺槐豆胶制成，以甘油作为增塑剂，并含有通过基于Box-Behnken设计的响应面法配制的CAE，用于设计、确定独立因素对拉伸强度的影响以及优化水凝胶贴剂配方。使用改良的Franz扩散池进行释放和皮肤渗透研究，并评估抗炎活性。
优化后的CAE水凝胶贴剂在预测和观察到的拉伸强度值之间显示出良好的相关性，其最大累积石蒜碱释放率和渗透率分别为69.38±2.78%和48.51±0.45%，符合Higuchi动力学模型。发现释放速率随着角叉菜胶和刺槐豆胶聚合物比例的增加而降低。此外，该贴剂具有强大的抗炎活性，IC值为21.36±0.78μg/mL。
成功制备了优化后的CAE水凝胶贴剂，具有优异的机械性能、累积释放、渗透和抗炎效果。因此，它有潜力进一步开发成为一种缓解疼痛和炎症的草药应用。该给药系统的抗炎作用应进一步研究。
