Inan Yuksel Esma, Cicek Demet, Demir Betul, Sahin Kazim, Tuzcu Mehmet, Orhan Cemal, Ozercan Ibrahim Hanifi, Sahin Fikrettin, Kocak Pelin, Yildirim Merve
Department of Dermatology, Biruni University Hospital, Istanbul, TUR.
Department of Dermatology, Faculty of Medicine, Firat University, Elazig, TUR.
Cureus. 2023 Jul 19;15(7):e42142. doi: 10.7759/cureus.42142. eCollection 2023 Jul.
Background Exosomes are membrane-derived nanovesicles produced by cells and play an important role in intercellular communication. Objectives This study aimed to investigate the effects of garlic exosome (GE) on hair growth. Methods Forty-two Sprague-Dawley/Wistar albino rats were randomly divided into six groups: non-shaved control, shaved control, topical control, GE 2 mg, GE 4 mg, and topical GE. At the end of the experiment, the number of hair follicles, follicle diameter, and subcutaneous tissue thicknesses were measured histopathologically. The Wnt-1, β-catenin, platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1), and collagen I levels were measured by the Western Blot method. Results The anagen follicle counts of the GE 2 mg, 4 mg, and topical GE groups were 66.57±15.49, 105.71±25.06, and 55.29±6.72, and were significantly higher than the control groups (p<0.01, p<0.001 and p<0.05, respectively). The follicle diameter of the GE 4 mg group was higher than the others (p<0.05). The Wnt-1, PDGF, VEGF, TGF-β1, and collagen I levels of all GE groups, and the β-catenin levels of the GE 4 mg and topical GE groups were significantly higher than the control groups (p<0.05). Conclusion GE induces hair growth in rats via the Wnt-1, β-catenin, VEGF, PDGF, and TGF-β1 signaling pathways.
背景 外泌体是细胞产生的膜源纳米囊泡，在细胞间通讯中发挥重要作用。目的 本研究旨在探讨大蒜外泌体（GE）对毛发生长的影响。方法 将42只Sprague-Dawley/Wistar白化大鼠随机分为六组：未剃毛对照组、剃毛对照组、局部对照组、GE 2mg组、GE 4mg组和局部GE组。实验结束时，通过组织病理学测量毛囊数量、毛囊直径和皮下组织厚度。采用蛋白质免疫印迹法检测Wnt-1、β-连环蛋白、血小板衍生生长因子（PDGF）、血管内皮生长因子（VEGF）、转化生长因子-β1（TGF-β1）和I型胶原蛋白水平。结果 GE 2mg组、4mg组和局部GE组的生长期毛囊计数分别为66.57±15.49、105.71±25.06和55.29±6.72，显著高于对照组（分别为p<0.01、p<0.001和p<0.05）。GE 4mg组的毛囊直径高于其他组（p<0.05）。所有GE组的Wnt-1、PDGF、VEGF、TGF-β1和I型胶原蛋白水平，以及GE 4mg组和局部GE组的β-连环蛋白水平均显著高于对照组（p<0.05）。结论 GE通过Wnt-1、β-连环蛋白、VEGF、PDGF和TGF-β1信号通路诱导大鼠毛发生长。
