Department of NanoEngineering, Chemical Engineering Program, and Moores Cancer Center, University of California San Diego, La Jolla, California 92093, United States.
Nano Lett. 2023 Sep 13;23(17):7941-7949. doi: 10.1021/acs.nanolett.3c01775. Epub 2023 Aug 21.
Cell membrane-based nanovaccines have demonstrated attractive features due to their inherently multiantigenic nature and ability to be formulated with adjuvants. Here, we report on cellular nanodiscs fabricated from cancer cell membranes and incorporated with a lipid-based adjuvant for antitumor vaccination. The cellular nanodiscs, with their small size and discoidal shape, are readily taken up by antigen-presenting cells and drain efficiently to the lymph nodes. Due to its highly immunostimulatory properties, the nanodisc vaccine effectively stimulates the immune system and promotes tumor-specific immunity. Using a murine colorectal cancer model, strong control of tumor growth is achieved in both prophylactic and therapeutic settings, particularly in combination with checkpoint blockades. Considerable therapeutic efficacy is also observed in treating a weakly immunogenic metastatic melanoma model. This work presents a new paradigm for the design of multiantigenic nanovaccines that can effectively activate antitumor immune responses and may be applicable to a wide range of cancers.
基于细胞膜的纳米疫苗因其固有多抗原性和与佐剂联合应用的能力而具有吸引力。在这里，我们报告了由癌细胞膜制备的细胞纳米盘，并与基于脂质的佐剂结合用于抗肿瘤疫苗接种。这些细胞纳米盘具有小尺寸和盘状形状，易于被抗原呈递细胞摄取，并有效地引流到淋巴结。由于其高度免疫刺激性，纳米盘疫苗有效地刺激免疫系统并促进肿瘤特异性免疫。在使用小鼠结直肠癌模型时，无论是在预防还是治疗环境中，都能实现对肿瘤生长的强烈控制，尤其是与检查点阻断联合使用时。在治疗弱免疫原性转移性黑色素瘤模型时，也观察到相当大的治疗效果。这项工作提出了一种新的多抗原纳米疫苗设计范式，能够有效激活抗肿瘤免疫反应，可能适用于广泛的癌症。
