Neurological Rehabilitation Center, Beijing Rehabilitation Hospital Affiliated to Capital Medical University, Xixiazhuang, Badachu, Shijingshan District, Beijing, China.
Medicine (Baltimore). 2023 Aug 18;102(33):e34706. doi: 10.1097/MD.0000000000034706.
Ischemic stroke is caused by insufficient blood supply to the brain. It has acute onset, often disturbance of consciousness, and high mortality and disability rate. However, relationship between ribosomal proteins (RP)-S15 and mitochondrial ribosomal proteins (MRP)-S27 and ischemic stroke remains unclear. The ischemic stroke datasets GSE22255, GSE16561, and GSE199435 were downloaded from gene expression omnibus generated by GPL6883, GPL11154, and GPL570. Differentially expressed genes (DEGs) were screened, and the construction and analysis of protein-protein interaction network, functional enrichment analysis and gene set enrichment analysis were performed. The gene expression heat map was drawn. Comparative toxicogenomics database analysis were performed to find the disease most related to core gene. TargetScan screened miRNAs that regulated central DEGs. Five hundred DEGs were identified. According to gene ontology analysis, they were mainly enriched in leukocyte activation, myoid cell activation involved in immune response, cell membrane, mitochondria, secretory vesicles, catalytic activity, enzyme binding, ribonucleic acid binding, splicing. Gene set enrichment analysis showed that the enrichment items are similar to the enrichment items of differentially expressed genes. And 20 core genes were obtained. Comparative toxicogenomics database analysis showed that 6 genes (RPS15, RPS2, RPS3, MRPS27, POLR2A, MRPS26) were found to be associated with chemical and drug-induced liver injury, necrosis, delayed prenatal exposure, nephropathy, hepatomegaly and tumor. RPS15 and MRPS27 are the core genes of ischemic stroke and play an important role in ischemic stroke.
缺血性脑卒中是由于脑的血液供应不足引起的。它具有发病急、常伴有意识障碍等特点，且死亡率和致残率较高。然而，核糖体蛋白（RP）-S15 和线粒体核糖体蛋白（MRP）-S27 与缺血性脑卒中之间的关系尚不清楚。从基因表达综合数据库中下载了 GSE22255、GSE16561 和 GSE199435 这三个缺血性脑卒中数据集，它们分别由 GPL6883、GPL11154 和 GPL570 产生。筛选差异表达基因（DEGs），构建和分析蛋白质-蛋白质相互作用网络，进行功能富集分析和基因集富集分析。绘制基因表达热图。对比较毒理学基因组数据库进行分析，以找到与核心基因最相关的疾病。TargetScan 筛选调控中心 DEGs 的 miRNAs。鉴定出 500 个 DEGs。根据基因本体论分析，它们主要富集在白细胞激活、参与免疫反应的肌细胞激活、细胞膜、线粒体、分泌小泡、催化活性、酶结合、核糖核酸结合、剪接。基因集富集分析显示，富集项目与差异表达基因的富集项目相似。并获得 20 个核心基因。比较毒理学基因组数据库分析显示，有 6 个基因（RPS15、RPS2、RPS3、MRPS27、POLR2A、MRPS26）与化学和药物诱导的肝损伤、坏死、产前暴露延迟、肾病、肝肿大和肿瘤有关。RPS15 和 MRPS27 是缺血性脑卒中的核心基因，在缺血性脑卒中的发生发展中起重要作用。
