Department of Anesthesiology, The Third Affiliated Hospital of Beijing University of Chinese Medicine, Anwai, Chaoyang District, Beijing, China.
Department of Disease Control and Prevention, The Third Affiliated Hospital of Beijing University of Chinese Medicine, Anwai, Chaoyang District, Beijing, China.
Medicine (Baltimore). 2024 Mar 1;103(9):e37123. doi: 10.1097/MD.0000000000037123.
Ketamine is the only intravenous narcotic that has sedative, analgesic, and anesthetic effects. However, the role of Flt3l and ribosomal protein S15 (Rps15) in ketamine anesthesia remains unclear. GSE26364 and GSE93041 were downloaded from gene expression omnibus. Multiple datasets were merged and batched. Differentially expressed genes (DEGs) were screened and weighted gene co-expression network analysis was performed. Construction and analysis of protein-protein interaction network. Gene ontology (GO) and Kyoto Encyclopedia of Gene and Genome were performed. A heat map of gene expression was drawn. TargetScan was used to screen miRNAs regulating DEGs. 882 DEGs were identified. According to the GO analysis, these DEGs were mainly enriched in cell differentiation, extracellular region, and cytoplasm. The Kyoto Encyclopedia of Gene and Genome analysis revealed enrichment in pathways such as the PPAR signaling pathway, TNF signaling pathway, Hippo signaling pathway, and IL-17 signaling pathway. In the Metascape enrichment analysis, GO enrichment categories included leukocyte differentiation, negative regulation of CREB transcription factor activity, and positive regulation of cell cycle. The protein-protein interaction network showed 10 core genes (Rpl7, Rpl18, Rps15, Rpl7l1, Flt3l, Rps16, Eprs, Rps19, Rps28, Rplp2).Gene expression heatmap showed that core genes (Rplp2, Flt3l, Rps15) were highly expressed in samples treated with ketamine anesthesia. Flt3l and Rps15 are highly expressed during ketamine anesthesia, and may be molecular targets.
氯胺酮是唯一具有镇静、镇痛和麻醉作用的静脉麻醉剂。然而，Flt3l 和核糖体蛋白 S15（Rps15）在氯胺酮麻醉中的作用尚不清楚。从基因表达综合数据库中下载 GSE26364 和 GSE93041。合并和分批处理多个数据集。筛选差异表达基因（DEGs），进行加权基因共表达网络分析。构建和分析蛋白质-蛋白质相互作用网络。进行基因本体论（GO）和京都基因与基因组百科全书分析。绘制基因表达热图。使用 TargetScan 筛选调节 DEGs 的 miRNAs。鉴定出 882 个 DEGs。根据 GO 分析，这些 DEGs 主要富集在细胞分化、细胞外区和细胞质中。京都基因与基因组百科全书分析显示，PPAR 信号通路、TNF 信号通路、Hippo 信号通路和 IL-17 信号通路等途径富集。在 Metascape 富集分析中，GO 富集类别包括白细胞分化、CREB 转录因子活性的负调控和细胞周期的正调控。蛋白质-蛋白质相互作用网络显示 10 个核心基因（Rpl7、Rpl18、Rps15、Rpl7l1、Flt3l、Rps16、Eprs、Rps19、Rps28、Rplp2）。基因表达热图显示，核心基因（Rplp2、Flt3l、Rps15）在氯胺酮麻醉处理的样本中高表达。Flt3l 和 Rps15 在氯胺酮麻醉期间高表达，可能是分子靶点。
