Leibniz Institute of Photonic Technology (Leibniz IPHT), Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), PO Box 100239, 07702, Jena, Germany.
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany.
Sci Rep. 2023 Aug 23;13(1):13779. doi: 10.1038/s41598-023-40944-6.
Here, we report on the development and application of a compact multi-core fiber optical probe for multimodal non-linear imaging, combining the label-free modalities of Coherent Anti-Stokes Raman Scattering, Second Harmonic Generation, and Two-Photon Excited Fluorescence. Probes of this multi-core fiber design avoid moving and voltage-carrying parts at the distal end, thus providing promising improved compatibility with clinical requirements over competing implementations. The performance characteristics of the probe are established using thin cryo-sections and artificial targets before the applicability to clinically relevant samples is evaluated using ex vivo bulk human and porcine intestine tissues. After image reconstruction to counteract the data's inherently pixelated nature, the recorded images show high image quality and morpho-chemical conformity on the tissue level compared to multimodal non-linear images obtained with a laser-scanning microscope using a standard microscope objective. Furthermore, a simple yet effective reconstruction procedure is presented and demonstrated to yield satisfactory results. Finally, a clear pathway for further developments to facilitate a translation of the multimodal fiber probe into real-world clinical evaluation and application is outlined.
在这里，我们报告了一种紧凑的多芯光纤光学探头的开发和应用，用于多模态非线性成像，结合了无标记模式的相干反斯托克斯拉曼散射、二次谐波产生和双光子激发荧光。这种多芯光纤设计的探头避免了在远端使用移动和带电压的部件，因此与竞争实现相比，具有更好的临床兼容性。该探头的性能特征是使用薄的冷冻切片和人工目标来建立的，然后使用离体的人体和猪肠组织来评估其在临床相关样本中的适用性。在对数据的固有像素化性质进行图像重建后，与使用标准显微镜物镜的激光扫描显微镜获得的多模态非线性图像相比，记录的图像在组织水平上显示出更高的图像质量和形态化学一致性。此外，还提出并演示了一种简单而有效的重建程序，以获得令人满意的结果。最后，概述了进一步开发的明确途径，以促进多模态光纤探头转化为实际的临床评估和应用。
