Kołakowska Anna, Kołbuk Dorota, Chwojnowski Andrzej, Rafalski Andrzej, Gadomska-Gajadhur Agnieszka
Faculty of Chemistry, Warsaw University of Technology, Noakowskiego St. 3, 00-664 Warsaw, Poland.
Institute of Fundamental Technological Research Polish Academy of Sciences, Pawińskiego St. 5B, 02-106 Warsaw, Poland.
J Funct Biomater. 2023 Aug 3;14(8):410. doi: 10.3390/jfb14080410.
An innovative approach to treating bone defects is using synthetic bone substitutes made of biomaterials. The proposed method to obtain polylactide scaffolds using the phase inversion technique with a freeze extraction variant enables the production of substitutes with morphology similar to cancellous bone (pore size 100-400 µm, open porosity 94%). The high absorbability of the implants will enable their use as platelet-rich plasma (PRP) carriers in future medical devices. Surface modification by dipping enabled the deposition of the hydrophilic chitosan (CS) layer, maintaining good bone tissue properties and high absorbability (850% dry weight). Introducing CS increases surface roughness and causes local changes in surface free energy, promoting bone cell adhesion. Through this research, we have developed a new and original method of low-temperature modification of PLA substitutes with chitosan. This method uses non-toxic reagents that do not cause changes in the structure of the PLA matrix. The obtained bone substitutes are characterised by exceptionally high hydrophilicity and morphology similar to spongy bone. In vitro studies were performed to analyse the effect of morphology and chitosan on cellular viability. Substitutes with properties similar to those of cancellous bone and which promote bone cell growth were obtained.
一种治疗骨缺损的创新方法是使用由生物材料制成的合成骨替代物。所提出的采用冷冻萃取变体的相转化技术来制备聚乳酸支架的方法，能够生产出形态与松质骨相似的替代物（孔径100 - 400 µm，开孔率94%）。植入物的高吸收性将使其能够在未来的医疗设备中用作富含血小板血浆（PRP）的载体。通过浸渍进行表面改性能够沉积亲水性壳聚糖（CS）层，保持良好的骨组织性能和高吸收性（干重的850%）。引入CS会增加表面粗糙度并导致表面自由能的局部变化，从而促进骨细胞粘附。通过这项研究，我们开发了一种用壳聚糖对聚乳酸替代物进行低温改性的全新原创方法。该方法使用无毒试剂，不会引起聚乳酸基质结构的变化。所获得的骨替代物具有极高的亲水性和与海绵骨相似的形态。进行了体外研究以分析形态和壳聚糖对细胞活力的影响。获得了具有与松质骨相似特性且能促进骨细胞生长的替代物。
