Laboratory of Molecular Biology, Centre for Innovative Research in Medical and Natural Sciences, Medical College of Rzeszow University, Warzywna 1a, 35-959 Rzeszow, Poland.
Institute of Physical Culture Sciences, Medical College of Rzeszow University, Cicha 2a, 35-959 Rzeszow, Poland.
Genes (Basel). 2023 Jul 27;14(8):1541. doi: 10.3390/genes14081541.
The gene plays a role in energy expenditure by participating in lipolysis, which affects body composition and performance. The rs4994 polymorphism has been studied in groups of athletes, overweight individuals, and obese and diabetic patients, but it has not been studied in young and healthy adults so far. In the present study, we examined the association of rs4994 polymorphism with body composition, somatotype, cardiorespiratory fitness and physical activity in young, healthy adults ( = 304). All subjects had anthropometric measurements, and somatotypes were assessed using the Heath-Carter method. In addition, cardiorespiratory fitness and physical activity levels were assessed. Genotyping for the gene was performed using a PCR-RFLP method. In the male group, body components were associated with the Trp64Trp genotype (waist circumference ( = 0.035), hip circumference ( = 0.029), BF (%) ( = 0.008), and BF (kg) ( = 0.010), BMI ( = 0.005), WHtR ( = 0.021), and BAI ( = 0.006)). In addition, we observed that the Trp64Trp genotype was associated with somatotype components ( = 0.013). In contrast, the Arg allele was associated with the ectomorphic components (0.006). We also observed a positive impact of the Trp64Trp genotype with maximal oxygen uptake (= 0.023) and oxygen pulse ( = 0.024). We observed a negative relationship of the Trp64Trp genotype in the female group with reported moderate-intensity exercise ( = 0.036). In conclusion, we found an association of the Trp64 allele with anthropometric traits, somatotype and parameters describing physical performance in the male group. In the female subpopulation, we only found an effect of the polymorphism Trp64Arg on the level of physical activity for moderate-intensity exercise.
该基因通过参与脂肪分解在能量消耗中发挥作用，脂肪分解会影响身体成分和表现。已经在运动员、超重个体、肥胖和糖尿病患者群体中研究了 rs4994 多态性，但到目前为止尚未在年轻健康成年人中进行研究。在本研究中，我们检查了 rs4994 多态性与年轻健康成年人（n=304）的身体成分、体型、心肺功能适应性和身体活动的关系。所有受试者均进行了人体测量，体型采用 Heath-Carter 方法评估。此外，还评估了心肺功能适应性和身体活动水平。基因分型使用 PCR-RFLP 方法进行。在男性组中，身体成分与 Trp64Trp 基因型相关（腰围（ = 0.035）、臀围（ = 0.029）、体脂百分比（ = 0.008）和体脂（kg）（ = 0.010）、BMI（ = 0.005）、WHtR（ = 0.021）和 BAI（ = 0.006））。此外，我们观察到 Trp64Trp 基因型与体型成分相关（ = 0.013）。相反，Arg 等位基因与外胚层成分相关（0.006）。我们还观察到 Trp64Trp 基因型与最大摄氧量呈正相关（= 0.023）和氧脉冲呈正相关（= 0.024）。在女性组中，我们观察到 Trp64Trp 基因型与报告的中等强度运动呈负相关（ = 0.036）。总之，我们发现 Trp64 等位基因与男性组的人体测量特征、体型和身体表现参数相关。在女性亚群中，我们仅发现 Trp64Arg 多态性对中等强度运动的身体活动水平有影响。
