Division of Molecular Biology, Interdisciplinary Institute of Indian System of Medicine (IIISM), SRM Institute of Science and Technology, Kattankulathur, India.
Centre for Ocean Research, Sathyabama Institute of Science and Technology, Chennai, India.
J Food Biochem. 2022 Aug;46(8):e14170. doi: 10.1111/jfbc.14170. Epub 2022 Apr 11.
Triterpenes and phytosterols enriched herbal formulations are known for glucose regulation and lipid metabolism. In this study, triterpenes and phytosterols from Moringa oleifera stem bark have been tested for their role in adipocyte differentiation. Chromatographic analysis revealed a wide range of phenolics, highlighting the presence of flavonoids (kaempferol, quercetin, and rutin), terpenoids (lupeol), and phytosterol (stigmasterol, β-sitosterol). Lupeol and β-sitosterol reduced cell viability in a dose-dependent manner showcasing increased G phase cell accumulation while reducing other cell cycle phases (S and G /M) and significant lowering of intracellular lipid accumulation. Additionally, lupeol (35.37% at 32 μM) and β-sitosterol (42.97% at 16 μM) inhibited reactive oxygen species generation and increased glucose uptake in adipocytes. Collectively, our results indicate that lupeol and β-sitosterol efficaciously attenuated adipogenesis via a controlled cell cycle progression and enhanced glucose uptake in adipocytes. PRACTICAL APPLICATIONS: Active components of Moringa oleifera effectively regulate adipocyte differentation suggest that it can be good medicial supllement for control of obesity.
三萜和植物甾醇丰富的草药配方以调节血糖和脂质代谢而闻名。在这项研究中，我们测试了辣木茎皮中的三萜和植物甾醇在脂肪细胞分化中的作用。色谱分析显示出广泛的酚类化合物，突出了类黄酮（山奈酚、槲皮素和芦丁）、萜类化合物（羽扇醇）和植物甾醇（豆甾醇、β-谷甾醇）的存在。羽扇醇和β-谷甾醇以剂量依赖性方式降低细胞活力，表现为 G 期细胞积累增加，同时减少其他细胞周期阶段（S 和 G /M），并显著降低细胞内脂质积累。此外，羽扇醇（在 32μM 时为 35.37%）和β-谷甾醇（在 16μM 时为 42.97%）抑制了脂肪细胞中活性氧的产生并增加了葡萄糖摄取。总的来说，我们的结果表明，羽扇醇和β-谷甾醇通过控制细胞周期进程有效地抑制脂肪生成，并增强脂肪细胞中的葡萄糖摄取。实际应用：辣木的有效活性成分调节脂肪细胞分化，表明它可以作为控制肥胖的良好药物补充剂。
