Cho Yoon Jae, Shin Bokyoung, Lee Sung-Ha, Park Sangmin, Kim Yoon-Keun, Kim Jae-Jin, Kim Eunjoo
Department of Psychiatry, College of Medicine, Yonsei University, Seoul 06273, Republic of Korea.
Center for Happiness Studies, Seoul National University, Seoul 08826, Republic of Korea.
Microorganisms. 2023 Aug 11;11(8):2063. doi: 10.3390/microorganisms11082063.
While interest in developing the human microbiome as a biomarker for attention-deficit hyperactivity disorder (ADHD) is increasing, there has been limited exploration in utilizing urine samples. In this study, we analysed urine microbiome profiles by extracting 16S ribosomal DNA from purified bacteria-derived extracellular membrane vesicles obtained from urine samples. Sequencing libraries were constructed by amplifying V3-V4 hypervariable regions sequenced using Illumina MiSeq. Profiles of male Korean children and adolescents with ADHD ( = 33) were compared with healthy sex-matched controls ( = 39). Statistically controlling for age, we found decreased alpha diversity in the urine bacteria of the ADHD group, as evidenced by reduced Shannon and Simpson indices ( < 0.05), and significant differences in beta diversity between the two groups ( < 0.001). The phyla and , as well as the genera and , were relatively more abundant in the ADHD group. The phylum and the genera and were more abundant in the control group. Notably, the genus exhibited significant correlations with the Child Behavior Checklist Attention Problems score and DSM-oriented ADHD subscale. This study is the first to propose the urine microbiome as a potential biomarker for pediatric ADHD.
虽然将人类微生物群开发为注意力缺陷多动障碍（ADHD）生物标志物的兴趣日益浓厚，但利用尿液样本进行的探索却很有限。在本研究中，我们通过从尿液样本中获得的纯化细菌衍生细胞外膜囊泡中提取16S核糖体DNA来分析尿液微生物群谱。通过扩增使用Illumina MiSeq测序的V3-V4高变区构建测序文库。将患有ADHD的韩国男性儿童和青少年（n = 33）的谱与健康的性别匹配对照（n = 39）进行比较。在对年龄进行统计学控制后，我们发现ADHD组尿液细菌中的α多样性降低，香农指数和辛普森指数降低证明了这一点（P < 0.05），并且两组之间的β多样性存在显著差异（P < 0.001）。ADHD组中的厚壁菌门和放线菌门以及葡萄球菌属和肠球菌属相对更为丰富。对照组中的拟杆菌门以及双歧杆菌属和罗氏菌属更为丰富。值得注意的是，葡萄球菌属与儿童行为检查表注意力问题得分和DSM导向的ADHD子量表表现出显著相关性。本研究首次提出尿液微生物群作为儿童ADHD的潜在生物标志物。
