Nguyen Tuong Ngoc-Gia, Pham Cuong Viet, Chowdhury Rocky, Patel Shweta, Jaysawal Satendra Kumar, Hou Yingchun, Xu Huo, Jia Lee, Duan Andrew, Tran Phuong Ha-Lien, Duan Wei
School of Medicine, Faculty of Health, Deakin University, Geelong Waurn Ponds Campus, Geelong, VIC 3216, Australia.
Laboratory of Tumor Molecular and Cellular Biology, College of Life Sciences, Shaanxi Normal University, 620 West Chang'an Avenue, Xi'an 710119, China.
Pharmaceutics. 2023 Aug 10;15(8):2115. doi: 10.3390/pharmaceutics15082115.
Over the past decade, there has been a significant expansion in the development of plant-derived extracellular nanovesicles (EVs) as an effective drug delivery system for precision therapy. However, the lack of effective methods for the isolation and characterization of plant EVs hampers progress in the field. To solve a challenge related to systemic separation and characterization in the plant-derived EV field, herein, we report the development of a simple 3D inner filter-based method that allows the extraction of apoplastic fluid (AF) from blueberry, facilitating EV isolation as well as effective downstream applications. Class I chitinase (PR-3) was found in blueberry-derived EVs (BENVs). As Class I chitinase is expressed in a wide range of plants, it could serve as a universal marker for plant-derived EVs. Significantly, the BENVs exhibit not only higher drug loading capacity than that reported for other EVs but also possess the ability to modulate the release of the proinflammatory cytokine IL-8 and total glutathione in response to oxidative stress. Therefore, the BENV is a promising edible multifunctional nano-bio-platform for future immunomodulatory therapies.
在过去十年中，作为一种用于精准治疗的有效药物递送系统，植物源细胞外纳米囊泡（EVs）的开发有了显著扩展。然而，缺乏有效的植物EVs分离和表征方法阻碍了该领域的进展。为了解决植物源EVs领域中与系统分离和表征相关的挑战，在此，我们报告了一种基于简单三维内滤器的方法的开发，该方法可从蓝莓中提取质外体流体（AF），有助于EVs的分离以及有效的下游应用。在蓝莓衍生的EVs（BENVs）中发现了I类几丁质酶（PR-3）。由于I类几丁质酶在多种植物中表达，它可作为植物源EVs的通用标记。重要的是，BENVs不仅显示出比其他报道的EVs更高的载药能力，而且具有响应氧化应激调节促炎细胞因子IL-8和总谷胱甘肽释放的能力。因此，BENV是未来免疫调节治疗中一种有前景的可食用多功能纳米生物平台。
