Department of Hepatobiliary Surgery, Shaoxing People's Hospital, Shaoxing, Zhejiang, China.
Department of Medicine, Shaoxing University School, Shaoxing, Zhejiang, China.
Technol Cancer Res Treat. 2023 Jan-Dec;22:15330338231198347. doi: 10.1177/15330338231198347.
Colorectal cancer is one of the most common causes of cancer-related death, and its main site of metastasis is the liver. The surgical method used for metastases of colorectal cancer in the liver varies according to the lobe affected, as does the prognosis. However, there is a lack of relevant basic research. Therefore, a good animal model is needed for basic studies of metastases from colorectal cancer to the different lobes of the liver.
A CT26 colon cancer cell line transfected with a virus expressing green fluorescent protein was inoculated into BALB/C mice via the spleen. Tumor formation in the liver lobes was observed under a fluorescence microscope according to which portal vein branch was ligated and according to clamping time. The differential formation of metastatic lesions in the different lobes was then compared with physical anatomy. Serum samples were used to detect the changes in liver function postoperatively.
Ligation and resection of the spleen 1 min after injection of the CT26 cells and release of the vessel clamp 1 min after splenectomy created an ideal tumor-bearing mouse model with little effect on liver function. Selective clamping of each portal vein branch and splenic injection of a CT26 cell line successfully established a selective liver lobe tumor-bearing model of colorectal cancer with distinct characteristics.
This model provides an opportunity for investigation of the mechanisms of metastasis of colorectal cancer to different lobes of the liver and may provide a basis for clinical treatment.
结直肠癌是癌症相关死亡的最常见原因之一，其主要转移部位是肝脏。用于治疗结直肠癌肝转移的手术方法因受影响的肝叶而异，预后也因肝叶而异。然而，相关基础研究却相对缺乏。因此，需要一种良好的动物模型，以进行结直肠癌转移到肝脏不同叶的基础研究。
通过脾脏将表达绿色荧光蛋白的病毒转染的 CT26 结肠癌细胞系接种到 BALB/C 小鼠体内。根据结扎的门静脉分支和夹闭时间，通过荧光显微镜观察肝叶中的肿瘤形成情况。然后，将不同叶转移性病变的差异形成与物理解剖进行比较。使用血清样本检测术后肝功能的变化。
在注射 CT26 细胞后 1 分钟结扎和切除脾脏，并在脾切除术 1 分钟后松开血管夹，可创建肝功能影响较小的理想荷瘤小鼠模型。选择性夹闭每个门静脉分支和脾内注射 CT26 细胞系可成功建立具有明显特征的结直肠癌选择性肝叶荷瘤模型。
该模型为研究结直肠癌向肝脏不同叶转移的机制提供了机会，并可能为临床治疗提供依据。
