Wang Fengqin, Gong Tao, Du Man, Xiao Xiao, Jiang Zipeng, Hu Weilian, Wang Yizhen, Cheng Yuanzhi
Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Hangzhou, 310058, China.
Key Laboratory of Animal Nutrition and Feed Science (Eastern of China), Ministry of Agriculture and Rural Affairs, Hangzhou, 310058, China.
AMB Express. 2023 Sep 4;13(1):93. doi: 10.1186/s13568-023-01598-9.
The biosynthetic process of selenium nanoparticles (SeNPs) by specific bacterial strain, whose growth directly affects the synthesis efficiency, has attracted great attentions. We previously reported that Bacillus paralicheniformis SR14, a SeNPs-producing bacteria, could improve intestinal antioxidative function in vitro. To further analyze the biological characteristics of SR14, whole genome sequencing was used to reveal the genetic characteristics in selenite reduction and sugar utilization. The results reviewed that the genome size of SR14 was 4,448,062 bp, with a GC content of 45.95%. A total of 4300 genes into 49 biological pathways was annotated to the KEGG database. EC: 1.1.1.49 (glucose-6-phosphate 1-dehydrogenase) and EC: 5.3.1.9 (glucose-6-phosphate isomerase), were found to play a potential role in glucose degradation and EC:2.7.1.4 (fructokinase) might be involved in the fructose metabolism. Growth profile and selenite-reducing ability of SR14 under different sugar supplements were determined and the results reviewed that glucose had a better promoting effect on the reduction of selenite and growth of bacteria than fructose, sucrose, and maltose. Moreover, RT-qPCR experiment proved that glucose supplement remarkably promoted the expressions of thioredoxin, fumarate reductase, and the glutathione peroxidase in SR14. Analysis of mRNA expression showed levels of glucose-6-phosphate dehydrogenase and fructokinase significantly upregulated under the supplement of glucose. Overall, our data demonstrated the genomic characteristics of SR14 and preliminarily determined that glucose supplement was most beneficial for strain growth and SeNPs synthesis.
特定细菌菌株生物合成硒纳米颗粒（SeNPs）的过程，其生长直接影响合成效率，已引起广泛关注。我们之前报道过，产SeNPs的细菌解淀粉芽孢杆菌SR14可在体外改善肠道抗氧化功能。为进一步分析SR14的生物学特性，利用全基因组测序揭示其在亚硒酸盐还原和糖利用方面的遗传特征。结果显示，SR14的基因组大小为4,448,062 bp，GC含量为45.95%。共有4300个基因被注释到KEGG数据库的49条生物学途径中。发现EC: 1.1.1.49（葡萄糖-6-磷酸脱氢酶）和EC: 5.3.1.9（葡萄糖-6-磷酸异构酶）在葡萄糖降解中可能发挥潜在作用，而EC:2.7.1.4（果糖激酶）可能参与果糖代谢。测定了SR14在不同糖补充条件下的生长曲线和亚硒酸盐还原能力，结果显示，与果糖、蔗糖和麦芽糖相比，葡萄糖对亚硒酸盐还原和细菌生长具有更好的促进作用。此外，RT-qPCR实验证明，补充葡萄糖显著促进了SR14中硫氧还蛋白、延胡索酸还原酶和谷胱甘肽过氧化物酶的表达。mRNA表达分析表明，在补充葡萄糖的情况下，葡萄糖-6-磷酸脱氢酶和果糖激酶的水平显著上调。总体而言，我们的数据展示了SR14的基因组特征，并初步确定补充葡萄糖对菌株生长和SeNPs合成最为有利。
