Sorbonne Université, Institut du Cerveau (ICM), Paris, France.
Methods Mol Biol. 2024;2707:125-140. doi: 10.1007/978-1-0716-3401-1_8.
Understanding how motor circuits are organized and recruited in order to perform complex behavior is an essential question of neuroscience. Here we present an optogenetic protocol on larval zebrafish that allows spatial selective control of neuronal activity within a genetically defined population. We combine holographic illumination with the use of effective opsin transgenic lines, alongside high-speed behavioral monitoring to dissect the motor circuits of the larval zebrafish.
了解运动回路是如何组织和募集的，以便执行复杂的行为，这是神经科学的一个基本问题。在这里，我们提出了一个在幼鱼斑马鱼上的光遗传学方案，该方案允许在遗传定义的群体内对神经元活性进行空间选择性控制。我们结合全息照明和有效的光遗传学转基因系的使用，以及高速行为监测，来剖析幼鱼斑马鱼的运动回路。
