Poostchi Ophthalmology Research Center, Department of Ophthalmology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Curr Stem Cell Res Ther. 2024;19(8):1111-1119. doi: 10.2174/1574888X19666230905114246.
The unique potential of stem cells to restore vision and regenerate damaged ocular cells has led to the increased attraction of researchers and ophthalmologists to ocular regenerative medicine in recent decades. In addition, advantages such as easy access to ocular tissues, non-invasive follow-up, and ocular immunologic privilege have enhanced the desire to develop ocular regenerative medicine.
This study aimed to characterize central and nasal orbital adipose stem cells (OASCs) and their neural differentiation potential.
The central and nasal orbital adipose tissues extracted during an upper blepharoplasty surgery were explant-cultured in Dulbecco's Modified Eagle Medium (DMEM)/F12 supplemented with 10% fetal bovine serum (FBS). Cells from passage 3 were characterized morphologically by osteogenic and adipogenic differentiation potential and by flow cytometry for expression of mesenchymal (CD73, CD90, and CD105) and hematopoietic (CD34 and CD45) markers. The potential of OASCs for the expression of , and and to induce neurogenesis was assessed by real-time PCR.
OASCs were spindle-shaped and positive for adipogenic and osteogenic induction. They were also positive for mesenchymal and negative for hematopoietic markers. They were positive for NGF expression in the absence of any significant alteration in the expression of and genes. Nasal OASCs had higher expression of CD90, higher potential for adipogenesis, a higher level of NGF expression under serum-free supplementation, and more potential for neuron-like morphology.
We suggested the explant method of culture as an easy and suitable method for the expansion of OASCs. Our findings denote mesenchymal properties of both central and nasal OASCs, while mesenchymal and neural characteristics were expressed stronger in nasal OASCs when compared to central ones. These findings can be added to the literature when cell transplantation is targeted in the treatment of neuro-retinal degenerative disorders.
干细胞具有恢复视力和再生受损眼部细胞的独特潜力，这使得近几十年来研究人员和眼科医生对眼部再生医学的兴趣大增。此外，易于获取眼部组织、非侵入性随访和眼部免疫特权等优势，增强了开发眼部再生医学的愿望。
本研究旨在描述中央和鼻侧眶脂肪干细胞（OASC）及其向神经分化的潜能。
在上眼睑成形术期间提取的中央和鼻侧眶脂肪组织在补充有 10%胎牛血清（FBS）的 Dulbecco 改良 Eagle 培养基（DMEM/F12）中进行组织块培养。第 3 代细胞通过成骨和成脂分化潜能以及通过流式细胞术检测间充质（CD73、CD90 和 CD105）和造血（CD34 和 CD45）标志物的表达来进行形态学特征描述。通过实时 PCR 评估 OASC 表达 、 和诱导神经发生的潜能。
OASC 呈纺锤形，对成脂和成骨诱导呈阳性。它们还对间充质标志物呈阳性，对造血标志物呈阴性。在没有任何明显改变 和 基因表达的情况下，它们对 NGF 表达呈阳性。鼻侧 OASC 表达 CD90 更高，成脂潜能更高，在无血清补充的情况下 NGF 表达水平更高，并且具有更强的神经元样形态潜能。
我们提出了组织块培养法作为一种简单且适合的 OASC 扩增方法。我们的研究结果表明，中央和鼻侧 OASC 具有间充质特性，而与中央 OASC 相比，鼻侧 OASC 具有更强的间充质和神经特性。这些发现可以为细胞移植治疗神经视网膜退行性疾病的治疗提供参考。
