采用高压液相色谱法测定血清和尿液中诺氟沙星和环丙沙星的浓度。
Determination of norfloxacin and ciprofloxacin concentrations in serum and urine by high-pressure liquid chromatography.
作者信息
Morton S J, Shull V H, Dick J D
出版信息
Antimicrob Agents Chemother. 1986 Aug;30(2):325-7. doi: 10.1128/AAC.30.2.325.
Abstract
A high-pressure liquid chromatographic method for the determination of norfloxacin or ciprofloxacin concentrations in body fluids was developed and compared with a standard bioassay. The high-pressure liquid chromatographic assay utilizes a reverse-phase C18 column, an internal standard, and fluorescence detection, with reproducibility studies yielding coefficients of variation ranging from 0.6 to 3.7% and 0.9 to 2.7% for norfloxacin and ciprofloxacin, respectively. Correlation coefficients with the bioassay were 0.966 for norfloxacin and 0.952 for ciprofloxacin.
摘要
开发了一种用于测定体液中诺氟沙星或环丙沙星浓度的高压液相色谱法，并与标准生物测定法进行了比较。高压液相色谱测定法采用反相C18柱、内标和荧光检测，重复性研究表明，诺氟沙星和环丙沙星的变异系数分别为0.6%至3.7%和0.9%至2.7%。与生物测定法的相关系数，诺氟沙星为0.966，环丙沙星为0.952。
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