Song Wanchen, Fang Zhongbiao, Ma Feike, Li Jiaxuan, Huang Zhiwei, Zhang Yanjun, Li Jianhua, Chen Keda
School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou, China.
Shulan International Medical College, Zhejiang Shuren University, Hangzhou, China.
Front Microbiol. 2023 Aug 14;14:1217567. doi: 10.3389/fmicb.2023.1217567. eCollection 2023.
Despite many countries rapidly revising their strategies to prevent contagions, the number of people infected with Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to surge. The emergent variants that can evade the immune response significantly affect the effectiveness of mainstream vaccines and diagnostic products based on the original spike protein. Therefore, it is essential to focus on the highly conserved nature of the nucleocapsid protein as a potential target in the field of vaccines and diagnostics. In this regard, our review initially discusses the structure, function, and mechanism of action of N protein. Based on this discussion, we summarize the relevant research on the in-depth development and application of diagnostic methods and vaccines based on N protein, such as serology and nucleic acid detection. Such valuable information can aid in designing more efficient diagnostic and vaccine tools that could help end the SARS-CoV-2 pandemic.
尽管许多国家迅速修订其预防传染病的策略，但感染严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的人数仍在激增。能够逃避免疫反应的新出现变种显著影响了基于原始刺突蛋白的主流疫苗和诊断产品的有效性。因此，将核衣壳蛋白的高度保守性质作为疫苗和诊断领域的潜在靶点至关重要。在这方面，我们的综述首先讨论了N蛋白的结构、功能和作用机制。基于此讨论，我们总结了基于N蛋白的诊断方法和疫苗（如血清学和核酸检测）深入开发和应用的相关研究。这些有价值的信息有助于设计更有效的诊断和疫苗工具，从而有助于终结SARS-CoV-2大流行。
