NeoCare, Reproduction, ENVT, Université de Toulouse, 23 Chemin des Capelles, BP 87614, 31 076, Toulouse Cedex 3, France.
Lallemand SAS, Blagnac, France.
Sci Rep. 2023 Sep 7;13(1):14717. doi: 10.1038/s41598-023-41422-9.
Low birth weight puppies present an increased risk of neonatal mortality, morbidity, and some long-term health issues. Yet it has not been investigated if those alterations could be linked to the gut microbiota composition and evolution. 57 puppies were weighed at birth and rectal swabs were performed at 5 time points from birth to 28 days of age. Puppies were grouped into three groups based on their birth weight: low birth weight (LBW), normal birth weight (NBW) and high birth weight (HBW). 16S rRNA gene sequencing was used to highlight differences in the fecal microbiota. During the first three weeks, the relative abundance of facultative anaerobic bacteria such as E. coli, C. perfringens and Tyzzerella was higher in LBW feces, but they catch back with the other groups afterwards. HBW puppies showed higher abundances of Faecalibacterium and Bacteroides during the neonatal period, suggesting an earlier maturation of their microbiota. The results of this study suggest that birth weight impact the initial establishment of the gut microbiota in puppies. Innovative strategies would be desired to deal with altered gut microbiota in low birth weight puppies aiming to improve their survival and long term health.
低出生体重的幼犬有更高的新生儿死亡率、发病率和一些长期健康问题的风险。然而，目前还没有研究这些改变是否与肠道微生物群的组成和演变有关。57 只幼犬在出生时进行称重，并在出生后至 28 天的 5 个时间点进行直肠拭子检查。根据出生体重，幼犬被分为三组：低出生体重（LBW）、正常出生体重（NBW）和高出生体重（HBW）。16S rRNA 基因测序用于突出粪便微生物群的差异。在前三周，LBW 粪便中兼性厌氧菌如大肠杆菌、产气荚膜梭菌和泰勒氏菌的相对丰度较高，但随后与其他组相持平。HBW 幼犬在新生儿期表现出更高的粪杆菌和拟杆菌丰度，这表明它们的微生物群更早成熟。这项研究的结果表明，出生体重影响幼犬肠道微生物群的初始建立。需要有创新的策略来处理低出生体重幼犬改变的肠道微生物群，以提高它们的存活率和长期健康。
