Scott Alison J, Ellis Shane R, Hofstaedter Casey E, Heeren Ron M A, Ernst Robert K
Department of Microbial Pathogenesis, University of Maryland School of Dentistry, Baltimore, MD 21201, USA.
Maastricht MultiModal Molecular Imaging (M4i) Institute, Maastricht University, 6200 MD Maastricht, the Netherlands.
iScience. 2023 Aug 21;26(9):107700. doi: 10.1016/j.isci.2023.107700. eCollection 2023 Sep 15.
() is a pathogen causing chronic pulmonary infections in patients with cystic fibrosis (CF). Manipulation of lipids is an important feature of infection and on a tissue-level scale is poorly understood. Using a mouse model of acute pulmonary infection, we explored the whole-lung phospholipid response using mass spectrometry imaging (MSI) and spatial lipidomics. Using a histology-driven analysis, we isolated airways and parenchyma from both mock- and -infected lungs and used systems biology tools to identify enriched metabolic pathways from the differential phospholipid identities. Infection was associated with a set of 26 ions, with 11 unique to parenchyma and 6 unique to airways. Acyl remodeling was differentially enriched in infected parenchyma as the predominant biological function. These functions correlated with markers of polymorphonuclear (PMN) cell influx, a defining feature of the lung response to infection, implicating enzymes active in phospholipid remodeling.
（）是一种导致囊性纤维化（CF）患者慢性肺部感染的病原体。脂质调控是感染的一个重要特征，而在组织水平上对此了解甚少。利用急性肺部感染小鼠模型，我们使用质谱成像（MSI）和空间脂质组学技术探索了全肺磷脂反应。通过组织学驱动的分析，我们从模拟感染和实际感染的肺部中分离出气道和实质，并使用系统生物学工具从差异磷脂特征中识别富集的代谢途径。感染与一组26种离子相关，其中11种是实质所特有的，6种是气道所特有的。酰基重塑在感染的实质中作为主要生物学功能差异富集。这些功能与多形核（PMN）细胞流入的标志物相关，这是肺部对感染反应的一个决定性特征，暗示了参与磷脂重塑的酶的作用。
