Institute of Laboratory Medicine, Universities of Giessen and Marburg Lung Center (UGMLC), Philipps University Marburg, German Center for Lung Research (DZL), 35043, Marburg, Germany.
Division of Pulmonary, Allergy and Critical Care Medicine, Sean N. Parker Center for Allergy and Asthma Research at Stanford University, Stanford, CA, 94040, USA.
Sci Rep. 2023 Sep 8;13(1):14874. doi: 10.1038/s41598-023-41187-1.
Respiratory virus infections play a major role in asthma, while there is a close correlation between asthma and food allergy. We hypothesized that T cell-mediated heterologous immunity may induce asthma symptoms among sensitized individuals and used two independent in silico pipelines for the identification of cross-reactive virus- and food allergen- derived T cell epitopes, considering individual peptide sequence similarity, MHC binding affinity and immunogenicity. We assessed the proteomes of human rhinovirus (RV1b), respiratory syncytial virus (RSVA2) and influenza-strains contained in the seasonal quadrivalent influenza vaccine 2019/2020 (QIV 2019/2020), as well as SARS-CoV-2 for human HLA alleles, in addition to more than 200 most common food allergen protein sequences. All resulting allergen-derived peptide candidates were subjected to an elaborate scoring system considering multiple criteria, including clinical relevance. In both bioinformatics approaches, we found that shortlisted peptide pairs that are potentially binding to MHC class II molecules scored up to 10 × lower compared to MHC class I candidate epitopes. For MHC class I food allergen epitopes, several potentially cross-reactive peptides from shrimp, kiwi, apple, soybean and chicken were identified. The shortlisted set of peptide pairs may be implicated in heterologous immune responses and translated to peptide immunization strategies with immunomodulatory properties.
呼吸道病毒感染在哮喘中起主要作用，而哮喘与食物过敏之间存在密切关联。我们假设 T 细胞介导的异源免疫可能在致敏个体中引起哮喘症状，并使用两种独立的计算管道来识别交叉反应性病毒和食物过敏原衍生的 T 细胞表位，考虑个体肽序列相似性、MHC 结合亲和力和免疫原性。我们评估了人类鼻病毒 (RV1b)、呼吸道合胞病毒 (RSVA2) 和包含在 2019/2020 季节性四价流感疫苗 (QIV 2019/2020) 中的流感株以及 SARS-CoV-2 对人类 HLA 等位基因的蛋白质组，以及 200 多种最常见的食物过敏原蛋白序列。所有由此产生的过敏原衍生肽候选物都经过了一个精心设计的评分系统的评估，该系统考虑了多个标准，包括临床相关性。在这两种生物信息学方法中，我们发现与 MHC 类 II 分子结合的短名单肽对与 MHC 类 I 候选表位相比得分低 10 倍。对于 MHC 类 I 食物过敏原表位，从虾、猕猴桃、苹果、大豆和鸡肉中鉴定出了几个潜在的交叉反应性肽。短名单肽对可能与异源免疫反应有关，并转化为具有免疫调节特性的肽免疫策略。
