Jung Jaeeun, Park Jeongjin, Lee Minhee, Kim Jinhak, Oh Dongchan, Jun Woojin, Kim Ok-Kyung, Lee Jeongmin
Department of Medical Nutrition, Kyung Hee University, Yongin 17104, Republic of Korea.
Division of Food and Nutrition and Human Ecology Research Institute, Chonnam National University, Gwangju 61186, Republic of Korea.
Foods. 2023 Aug 22;12(17):3149. doi: 10.3390/foods12173149.
The excessive storage of triglycerides in adipose tissue is a characteristic feature of obesity, which arises from an imbalance between energy intake and expenditure. In this study, we aimed to explore the potential anti-obesity effects of extracts (SC) in a high-fat diet (HFD)-induced in obese mice and 3T3-L1 adipocytes, with a specific focus on understanding the underlying lipid mechanisms. Mice were fed with a normal diet (NC; normal control), HFD (60% high-fat diet), Met (HFD containing metformin 250 mg/kg b.w.), SC25 (HFD containing SC 25 mg/kg b.w.), SC50 (HFD containing SC 50 mg/kg b.w.), or SC 100 (HFD containing SC 100 mg/kg b.w.) for 12 weeks. Notably, SC supplementation led to significant reductions in body weight gain, adipose tissue weight, adipose tissue mass, and adipocyte size in HFD-fed mice. Furthermore, SC supplementation exerted inhibitory effects on the adipogenesis and lipogenesis pathways while promoting lipolysis and thermogenesis pathways in the adipose tissues of HFD-fed mice. In vitro experiments using 3T3-L1 cells demonstrated that SC treatment during the differentiation phase suppressed adipogenesis and lipogenesis, whereas SC treatment after differentiation, activated lipolysis and thermogenesis. Collectively, these findings indicate that SC exhibits a direct influence on the lipid metabolism of adipocytes, making it an effective candidate for weight loss interventions.
脂肪组织中甘油三酯的过度储存是肥胖的一个特征，肥胖源于能量摄入与消耗之间的失衡。在本研究中，我们旨在探讨提取物（SC）对高脂饮食（HFD）诱导的肥胖小鼠和3T3-L1脂肪细胞的潜在抗肥胖作用，特别关注了解其潜在的脂质机制。给小鼠喂食正常饮食（NC；正常对照）、HFD（60%高脂饮食）、Met（含250 mg/kg体重二甲双胍的HFD）、SC25（含25 mg/kg体重SC的HFD）、SC50（含50 mg/kg体重SC的HFD）或SC 100（含100 mg/kg体重SC的HFD），持续12周。值得注意的是，补充SC可显著降低HFD喂养小鼠的体重增加、脂肪组织重量、脂肪组织质量和脂肪细胞大小。此外，补充SC对HFD喂养小鼠脂肪组织中的脂肪生成和脂质生成途径具有抑制作用，同时促进脂肪分解和产热途径。使用3T3-L1细胞的体外实验表明，在分化阶段进行SC处理可抑制脂肪生成和脂质生成，而在分化后进行SC处理则可激活脂肪分解和产热。总体而言，这些发现表明SC对脂肪细胞的脂质代谢具有直接影响，使其成为减肥干预的有效候选物。
