Department of Nanoengineering, University of California, San Diego, La Jolla, California 92093, United States.
J Am Chem Soc. 2020 Apr 1;142(13):5991-5995. doi: 10.1021/jacs.0c01883. Epub 2020 Mar 24.
There are urgent needs for sensing devices capable of distinguishing between episodes of opioid overdose and nerve agent poisoning. This work presents a wearable microneedle sensor array for minimally invasive continuous electrochemical detection of opioid (OPi) and organophosphate (OP) nerve agents on a single patch platform. The new multimodal microneedle sensor array relies on unmodified and organophosphorus hydrolase (OPH) enzyme-modified carbon paste (CP) microneedle electrodes for square wave voltammetric (SWV) detection of the fentanyl and nerve agent targets, respectively. Such real-time simultaneous sensing provides distinct unique information, along with attractive analytical performance, including high sensitivity, selectivity, and stability, for real-time on-body OPi-OP analysis. The patch represents the first sensing device capable of continuously monitoring fentanyl down to the nanomolar level through a nanomaterial-based multilayered surface architecture. Applicability of the sensor array toward opioids screening is demonstrated for morphine and norfentanyl. Successful OPi-OP detection conducted in a skin-mimicking phantom gel demonstrates the suitability of the device for rapid on-body sensing. Such progress toward continuous minimally invasive transdermal analysis of drugs of abuse and nerve agents holds promise for rapid countermeasures for protecting soldiers, civilians, and healthcare personnel.
对于能够区分阿片类药物过量和神经毒剂中毒发作的传感设备存在迫切需求。本工作提出了一种可穿戴的微针传感器阵列，用于在单个贴片平台上对阿片类药物（OPi）和有机磷（OP）神经毒剂进行微创连续电化学检测。新型多模态微针传感器阵列依赖于未经修饰和有机磷水解酶（OPH）酶修饰的碳糊（CP）微针电极，分别用于芬太尼和神经毒剂靶标的方波伏安法（SWV）检测。这种实时同时传感提供了独特的信息，同时具有吸引人的分析性能，包括高灵敏度、选择性和稳定性，可用于实时体内 OPi-OP 分析。该贴片代表了第一个能够通过基于纳米材料的多层表面结构连续监测纳摩尔级芬太尼的传感设备。该传感器阵列对吗啡和诺芬太尼的阿片类药物筛选的适用性进行了证明。在皮肤模拟凝胶中的成功 OPi-OP 检测证明了该设备适用于快速体内传感。这种朝着对滥用药物和神经毒剂进行连续微创透皮分析的进展有望为保护士兵、平民和医护人员提供快速对策。
