Department of Nanoengineering, University of California San Diego, La Jolla, CA, USA.
Department of Electrical and Computer Engineering, University of California San Diego, La Jolla, CA, USA.
Nat Biomed Eng. 2022 Nov;6(11):1214-1224. doi: 10.1038/s41551-022-00887-1. Epub 2022 May 9.
Implementations of wearable microneedle-based arrays of sensors for the monitoring of multiple biomarkers in interstitial fluid have lacked system integration and evidence of robust analytical performance. Here we report the development and testing of a fully integrated wearable array of microneedles for the wireless and continuous real-time sensing of two metabolites (lactate and glucose, or alcohol and glucose) in the interstitial fluid of volunteers performing common daily activities. The device works with a custom smartphone app for data capture and visualization, comprises reusable electronics and a disposable microneedle array, and is optimized for system integration, cost-effective fabrication via advanced micromachining, easier assembly, biocompatibility, pain-free skin penetration and enhanced sensitivity. Single-analyte and dual-analyte measurements correlated well with the corresponding gold-standard measurements in blood or breath. Further validation of the technology in large populations with concurrent validation of sensor readouts through centralized laboratory tests should determine the robustness and utility of real-time simultaneous monitoring of several biomarkers in interstitial fluid.
目前，用于监测细胞间液中多种生物标志物的可穿戴式微针传感器阵列在系统集成和稳健分析性能方面仍存在不足。在此，我们报告了一种完全集成的可穿戴式微针阵列的开发和测试，该阵列可用于无线和连续实时感测志愿者在进行日常活动时细胞间液中的两种代谢物（乳酸和葡萄糖，或酒精和葡萄糖）。该设备与自定义的智能手机应用程序配合使用，用于数据采集和可视化，由可重复使用的电子设备和一次性微针阵列组成，并针对系统集成、通过先进的微加工实现具有成本效益的制造、更简单的组装、生物相容性、无痛皮肤穿透和增强的灵敏度进行了优化。单一分析物和双分析物的测量结果与血液或呼吸中的相应金标准测量结果相关性良好。通过集中实验室测试对该技术在大人群中的进一步验证，以及对传感器读数的同时验证，应确定细胞间液中几种生物标志物的实时同步监测的稳健性和实用性。
