Zhao Liang, Wen Zhuangzhuang, Jiang Fujian, Zheng Zhaozhu, Lu Shenzhou
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering Renai Road, Suzhou Industrial Park Suzhou Jiangsu P. R. China 215123
RSC Adv. 2020 Feb 10;10(11):6163-6171. doi: 10.1039/c9ra10374k. eCollection 2020 Feb 7.
This work illustrates the feasibility of a microneedle based electrochemical biosensor for continuous glucose monitoring. The device consists of three silk/d-sorbitol pyramidal microneedles integrated with platinum (Pt) and silver (Ag) wires and immobilized glucose selective enzyme (glucose oxidase, GOD) during fabrication. The silk/d-sorbitol composite can provide a biocompatible environment for the enzyme molecules. The break strength can be controlled by the ratio of silk to d-sorbitol, which guarantees microneedle penetrate into skin. The enzymatic-amperometric responses and glucose concentration were linearly correlated, and cover physiological conditions. The microneedle displays high stability both in long-term monitoring and storage, even at 37 °C. Our results reveal that this new microneedle biosensor is a promising tool for wearable minimally invasive continuous glucose monitoring in practical applications.
这项工作说明了基于微针的电化学生物传感器用于连续血糖监测的可行性。该装置由三根集成了铂（Pt）和银（Ag）线的丝/山梨醇金字塔形微针组成，并在制造过程中固定了葡萄糖选择性酶（葡萄糖氧化酶，GOD）。丝/山梨醇复合材料可为酶分子提供生物相容的环境。断裂强度可通过丝与山梨醇的比例来控制，这确保了微针能够刺入皮肤。酶促安培响应与葡萄糖浓度呈线性相关，且涵盖生理条件。即使在37°C下，该微针在长期监测和储存中都表现出高稳定性。我们的结果表明，这种新型微针生物传感器在实际应用中是用于可穿戴微创连续血糖监测的一种有前途的工具。
