Huang Qing, Zhang Deli, Diao Qingchun, Lin Mao
Department of Dermatology, Chongqing Shapingba District People's Hospital, Chongqing 400011, P.R. China.
Department of Dermatology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400011, P.R. China.
Oncol Lett. 2019 Sep;18(3):2917-2922. doi: 10.3892/ol.2019.10631. Epub 2019 Jul 18.
The development of melanoma may involve long non-coding RNAs (lncRNAs); however, the functions of the majority of lncRNAs in melanoma are unknown. The present study investigated the role of long intergenic non-protein coding RNA p53 induced transcript (LINC-PINT) in melanoma. In the present study, quantitative PCR was used to detect gene expression, overexpression experiments were performed to analyze gene interactions and CCK-8 assays were used to analyze cell proliferation. LINC-PINT was downregulated, while BRAF-activated non-coding RNA (BANCR) was upregulated in melanoma tissues compared with normal adjacent tissues. Expression levels of LINC-PINT decreased, while expression levels of BANCR increased with increasing tumor thickness. The expression levels of LINC-PINT and BANCR were inversely associated in melanoma tissues but not in healthy adjacent tissue. LINC-PINT overexpression downregulated BANCR expression in melanoma cells, while BANCR overexpression did not significantly affect LINC-PINT expression. LINC-PINT overexpression inhibited melanoma cell proliferation compared to controls. BANCR overexpression attenuated the effects of LINC-PINT overexpression. The present study revealed that lncRNA LINC-PINT is downregulated in melanoma and may regulate melanoma cell proliferation by downregulating lncRNA BANCR.
黑色素瘤的发生发展可能涉及长链非编码RNA（lncRNA）；然而，大多数lncRNA在黑色素瘤中的功能尚不清楚。本研究调查了长链基因间非编码RNA p53诱导转录本（LINC-PINT）在黑色素瘤中的作用。在本研究中，采用定量PCR检测基因表达，进行过表达实验分析基因相互作用，并使用CCK-8法分析细胞增殖。与相邻正常组织相比，黑色素瘤组织中LINC-PINT表达下调，而BRAF激活的非编码RNA（BANCR）表达上调。随着肿瘤厚度增加，LINC-PINT表达水平降低，而BANCR表达水平升高。在黑色素瘤组织中，LINC-PINT和BANCR的表达水平呈负相关，但在相邻健康组织中并非如此。LINC-PINT过表达下调黑色素瘤细胞中BANCR的表达，而BANCR过表达对LINC-PINT表达无显著影响。与对照组相比，LINC-PINT过表达抑制黑色素瘤细胞增殖。BANCR过表达减弱了LINC-PINT过表达的作用。本研究表明，lncRNA LINC-PINT在黑色素瘤中表达下调，并可能通过下调lncRNA BANCR来调节黑色素瘤细胞增殖。
