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CCN2衍生的环状RNA在软骨细胞中的表达及功能

Expression and function of CCN2-derived circRNAs in chondrocytes.

作者信息

Kato Soma, Kawata Kazumi, Nishida Takashi, Mizukawa Tomomi, Takigawa Masaharu, Iida Seiji, Kubota Satoshi

机构信息

Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-Cho, Kita-Ku, Okayama, 700-8525, Japan.

Department of Oral Maxillofacial Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.

出版信息

J Cell Commun Signal. 2023 Dec;17(4):1501-1515. doi: 10.1007/s12079-023-00782-7. Epub 2023 Sep 11.

DOI:10.1007/s12079-023-00782-7
PMID:37695440
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10713908/
Abstract

Cellular communication network factor 2 (CCN2) molecules promote endochondral ossification and articular cartilage regeneration, and circular RNAs (circRNAs), which arise from various genes and regulate gene expression by adsorbing miRNAs, are known to be synthesized from CCN2 in human vascular endothelial cells and other types of cells. However, in chondrocytes, not only the function but also the presence of CCN2-derived circRNA remains completely unknown. In the present study, we investigated the expression and function of CCN2-derived circRNAs in chondrocytes. Amplicons smaller than those from known CCN2-derived circRNAs were observed using RT-PCR analysis that could specifically amplify CCN2-derived circRNAs in human chondrocytic HCS-2/8 cells. The nucleotide sequences of the PCR products indicated novel circRNAs in the HCS-2/8 cells that were different from known CCN2-derived circRNAs. Moreover, the expression of several Ccn2-derived circRNAs in murine chondroblastic ATDC5 cells was confirmed and observed to change alongside chondrocytic differentiation. Next, one of these circRNAs was knocked down in HCS-2/8 cells to investigate the function of the human CCN2-derived circRNA. As a result, CCN2-derived circRNA knockdown significantly reduced the expression of aggrecan mRNA and proteoglycan synthesis. Our data suggest that CCN2-derived circRNAs are expressed in chondrocytes and play a role in chondrogenic differentiation. Production and role of CCN2-derived RNAs in chondrocytes.

摘要

细胞通讯网络因子2（CCN2）分子可促进软骨内骨化和关节软骨再生，而环状RNA（circRNA）由多种基因产生，并通过吸附微小RNA（miRNA）来调控基因表达，已知其可在人血管内皮细胞和其他类型细胞中由CCN2合成。然而，在软骨细胞中，不仅CCN2衍生的circRNA的功能完全未知，其存在情况也完全不明。在本研究中，我们调查了CCN2衍生的circRNA在软骨细胞中的表达及功能。使用逆转录聚合酶链反应（RT-PCR）分析观察到，在人软骨细胞系HCS-2/8细胞中，存在比已知CCN2衍生的circRNA更小的扩增子，该分析能够特异性扩增CCN2衍生的circRNA。PCR产物的核苷酸序列表明，HCS-2/8细胞中存在与已知CCN2衍生的circRNA不同的新型circRNA。此外，在小鼠软骨母细胞系ATDC5细胞中证实了几种CCN2衍生的circRNA的表达，并观察到其表达随软骨细胞分化而变化。接下来，在HCS-2/8细胞中敲低其中一种circRNA，以研究人CCN2衍生的circRNA的功能。结果显示，敲低CCN2衍生的circRNA显著降低了聚集蛋白聚糖mRNA的表达和蛋白聚糖的合成。我们的数据表明，CCN2衍生的circRNA在软骨细胞中表达，并在软骨形成分化中发挥作用。CCN2衍生的RNA在软骨细胞中的产生及作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a4c/10713908/e62474301d1a/12079_2023_782_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a4c/10713908/a302c0e80c47/12079_2023_782_Figa_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a4c/10713908/477f00b77fea/12079_2023_782_Fig4_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a4c/10713908/57728655921e/12079_2023_782_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a4c/10713908/fa4c6925a03d/12079_2023_782_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a4c/10713908/e62474301d1a/12079_2023_782_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a4c/10713908/a302c0e80c47/12079_2023_782_Figa_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a4c/10713908/7beb1318d425/12079_2023_782_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a4c/10713908/7a530184805e/12079_2023_782_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a4c/10713908/d4d42b92e91e/12079_2023_782_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a4c/10713908/477f00b77fea/12079_2023_782_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a4c/10713908/4ee8f6406683/12079_2023_782_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a4c/10713908/57728655921e/12079_2023_782_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a4c/10713908/fa4c6925a03d/12079_2023_782_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a4c/10713908/e62474301d1a/12079_2023_782_Fig8_HTML.jpg

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Anat Rec (Hoboken). 2023 Dec;306(12):3006-3020. doi: 10.1002/ar.24930. Epub 2022 Apr 21.
2
Specific expression and functions of circular RNAs.环状RNA的特异性表达及功能
Cell Death Differ. 2022 Mar;29(3):481-491. doi: 10.1038/s41418-022-00948-7. Epub 2022 Feb 15.
3
CircRNA ciRS-7: a Novel Oncogene in Multiple Cancers.环状 RNA ciRS-7：多种癌症中的新型癌基因。
Int J Biol Sci. 2021 Jan 1;17(1):379-389. doi: 10.7150/ijbs.54292. eCollection 2021.
4
CircRNA inhibits DNA damage repair by interacting with host gene.环状 RNA 通过与宿主基因相互作用抑制 DNA 损伤修复。
Mol Cancer. 2020 Aug 24;19(1):128. doi: 10.1186/s12943-020-01246-x.
5
lncRNA MIAT increases cell viability, migration, EMT and ECM production in age-related cataracts by regulating the miR-181a/CTGF/ERK signaling pathway.长链非编码RNA MIAT通过调控miR-181a/结缔组织生长因子/细胞外信号调节激酶信号通路，增加年龄相关性白内障中的细胞活力、迁移能力、上皮-间质转化及细胞外基质生成。
Exp Ther Med. 2020 Aug;20(2):1053-1063. doi: 10.3892/etm.2020.8749. Epub 2020 May 13.
6
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7
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