Han Xu, Li Qian, Wei Hongli, Zheng Yingying, Shang Na, Chen Chunchi, Guo Reyting, Gao Jian, Liu Weidong
National Engineering Research Center for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
National Technology Innovation Center of Synthetic Biology, Tianjin 300308, China.
Sheng Wu Gong Cheng Xue Bao. 2022 Nov 25;38(11):4050-4067. doi: 10.13345/j.cjb.220596.
Repurpose of key catalytic reaction and reconstruction of metabolic pathways all depend on in-depth understanding of the relevant proteins in protein engineering, green biomanufacturing and synthetic biology. The rapid development of synthetic biotechnology calls for proteins with excellent performance to fulfill the requirement for engineering enzymes and strains. The key is to prepare large quantities of target proteins with high purity, and study the structure-function relationship quickly and accurately. In the past 10 years, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences has built a protein structure-function study platform, and studied many proteins of industrial significance. Progress has been made on the terpene synthase related to natural plant products synthesis, PET plastic degradation related enzyme, and enzymes involved in biomass conversion and utilization. The structure-function study of these proteins provided theoretical basis for further engineering. Development of protein structure-function research technologies will facilitate the research of synthetic biology and promote biomanufacturing.
关键催化反应的重新利用和代谢途径的重建均依赖于在蛋白质工程、绿色生物制造和合成生物学中对相关蛋白质的深入理解。合成生物技术的快速发展需要具有优异性能的蛋白质来满足对工程酶和菌株的要求。关键在于制备大量高纯度的目标蛋白质，并快速准确地研究其结构-功能关系。在过去十年中，中国科学院天津工业生物技术研究所建立了蛋白质结构-功能研究平台，并研究了许多具有工业意义的蛋白质。在与天然植物产物合成相关的萜类合酶、PET塑料降解相关酶以及参与生物质转化与利用的酶方面取得了进展。这些蛋白质的结构-功能研究为进一步的工程改造提供了理论依据。蛋白质结构-功能研究技术的发展将推动合成生物学研究并促进生物制造。
