绝经前和绝经后高级别浆液性卵巢癌腹水的单细胞图谱。
A single-cell landscape of pre- and post-menopausal high-grade serous ovarian cancer ascites.
作者信息
Chen Wenying, Liu Hanyuan, Huang Xinya, Qian Lili, Chen Liang, Zhou Yonggang, Liu Yi, Liu Yujie, Wang Yingying, Zhang Tianjiao, Zhou Youyang, Fang Jingwen, Yang Jiaxuan, Ni Fang, Guo Chuang, Zhou Ying
机构信息
Department of Obstetrics and Gynecology, Core Facility Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, China.
Department of Cardiology, The First Affiliated Hospital of USTC, the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Science and Medicine, University of Science and Technology of China, Hefei, Anhui, China.
出版信息
iScience. 2023 Aug 25;26(10):107712. doi: 10.1016/j.isci.2023.107712. eCollection 2023 Oct 20.
High-grade serous ovarian cancer (HGSOC) is a hormone-related cancer with high mortality and poor prognosis. Based on the transcriptome of 57,444 cells in ascites from 10 patients with HGSOC (including 5 pre-menopausal and 5 post-menopausal patients), we identified 14 cell clusters which were further classified into 6 cell types, including T cells, B cells, NK cells, myeloid cells, epithelial cells, and stromal cells. We discovered an increased proportion of epithelial cells and a decreased proportion of T cells in pre-menopausal ascites compared with post-menopausal ascites. GO analysis revealed the pre-menopausal tumor microenvironments (TME) are closely associated with viral infection, while the post-menopausal TME are mostly related to the IL-17 immune pathway. SPP1/CD44-mediated crosstalk between myeloid cells and B cells, NK cells, and stromal cells mainly present in the pre-menopausal group, while SPP1/PTGER4 -mediated crosstalk between myeloid cells and epithelial cells mostly present in the post-menopausal group.
高级别浆液性卵巢癌（HGSOC）是一种与激素相关的癌症，死亡率高且预后较差。基于10例HGSOC患者（包括5例绝经前和5例绝经后患者）腹水中57444个细胞的转录组，我们鉴定出14个细胞簇，这些细胞簇进一步分为6种细胞类型，包括T细胞、B细胞、NK细胞、髓样细胞、上皮细胞和基质细胞。我们发现，与绝经后腹水相比，绝经前腹水中上皮细胞比例增加，T细胞比例降低。基因本体（GO）分析显示，绝经前肿瘤微环境（TME）与病毒感染密切相关，而绝经后TME主要与白细胞介素-17免疫途径相关。SPP1/CD44介导的髓样细胞与B细胞、NK细胞和基质细胞之间的串扰主要存在于绝经前组，而SPP1/PTGER4介导的髓样细胞与上皮细胞之间的串扰主要存在于绝经后组。
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