定植于孕妇阴道的致外阴阴道念珠菌病的念珠菌属分离株的磷脂酶和蛋白酶活性。
Phospholipase and proteinase activities of isolates of colonizing Candida spp. causing vulvovaginitis in pregnant women.
机构信息
Grupo de Micología, Facultad de Medicina, Universidad de Cartagena, Cartagena, Colombia.
出版信息
Biomedica. 2022 Aug 31;43(Sp. 1):89-96. doi: 10.7705/biomedica.6759.
INTRODUCTION
Proteases and phospholipases are virulence factors of Candida spp. that play an important role in tissue invasion. Among the factors related to the host some are associated with environmental characteristics and others with Candida colonization.
OBJECTIVES
To determine phospholipase and protease activities in colonizing and pathogenic strains, isolated from pregnant women in Cartagena de Indias.
MATERIALS AND METHODS
Phospholipase and protease activity was determined in 56 isolates, evaluating substrate degradation and calculating the enzyme activity coefficient. Phospholipase and protease activities were compared between colonizing and pathogenic strains.
RESULTS
“Very high” (<0.69) phospholipase and protease activity was found in 34 and 14 isolates, respectively. There was no significant difference when comparing phospholipase and protease activities between colonizing and pathogenic isolates.
CONCLUSIONS
Phospholipase activity predominated as a virulence factor in the studied strains, but no significant difference found between colonizing and pathogenic strains for phospholipase and protease activities.
简介
蛋白酶和磷脂酶是假丝酵母菌属的毒力因子，在组织侵袭中起重要作用。与宿主有关的因素中，有些与环境特征有关，有些与假丝酵母菌定植有关。
目的
测定从卡塔赫纳市孕妇中分离出的定植和致病菌株的磷脂酶和蛋白酶活性。
材料和方法
对 56 株分离株进行了磷脂酶和蛋白酶活性测定，评估了底物降解并计算了酶活性系数。比较了定植和致病菌株的磷脂酶和蛋白酶活性。
结果
34 株和 14 株分离株的磷脂酶和蛋白酶活性分别为“非常高”（<0.69）。在比较定植和致病分离株的磷脂酶和蛋白酶活性时，没有发现显著差异。
结论
磷脂酶活性在研究菌株中作为毒力因子占主导地位，但在定植和致病菌株之间，磷脂酶和蛋白酶活性没有发现显著差异。
Zotero 插件