Department of Chemistry, University of Surrey, Guildford GU2 7XH, U.K.
Faculty of Health & Medical Sciences, University of Surrey, Guildford GU2 7XH, U.K.
Anal Chem. 2023 Oct 3;95(39):14727-14735. doi: 10.1021/acs.analchem.3c02854. Epub 2023 Sep 19.
In this work, we demonstrate the development and first application of nanocapillary sampling followed by analytical flow liquid chromatography-mass spectrometry for single-cell lipidomics. Around 260 lipids were tentatively identified in a single cell, demonstrating remarkable sensitivity. Human pancreatic ductal adenocarcinoma cells (PANC-1) treated with the chemotherapeutic drug gemcitabine can be distinguished from controls solely on the basis of their single-cell lipid profiles. Notably, the relative abundance of LPC(0:0/16:0) was significantly affected in gemcitabine-treated cells, in agreement with previous work in bulk. This work serves as a proof of concept that live cells can be sampled selectively and then characterized using automated and widely available analytical workflows, providing biologically relevant outputs.
在这项工作中，我们展示了纳米毛细管采样结合分析型流动液相色谱-质谱联用技术在单细胞脂质组学中的开发和首次应用。在单个细胞中，我们暂定鉴定了约 260 种脂质，这表明了该方法具有显著的灵敏度。仅基于其单细胞脂质谱，用化疗药物吉西他滨处理的人胰腺导管腺癌（PANC-1）细胞就可以与对照细胞区分开来。值得注意的是，与以往的批量研究一致，LPC（0：0/16：0）的相对丰度在吉西他滨处理的细胞中受到显著影响。这项工作验证了一种概念，即可以有选择地对活细胞进行采样，然后使用自动化和广泛可用的分析工作流程对其进行特征描述，从而获得具有生物学意义的结果。
