Universidade Federal Rio de Janeiro, Faculdade de Odontologia, Departamento de Clínica Odontológica, Rio de Janeiro, Brasil.
Universidade do Grande Rio, Programa de Pós-graduação em Odontologia, Duque de Caxias, Rio de Janeiro, Brasil.
J Appl Oral Sci. 2023 Sep 15;31:e20230134. doi: 10.1590/1678-7757-2023-0134. eCollection 2023.
To evaluate the colonization dynamics of subgingival microbiota established over six months around newly installed dental implants in periodontally healthy individuals, compared with their corresponding teeth.
Seventeen healthy individuals assigned to receive single dental implants participated in the study. Subgingival biofilm was sampled from all implant sites and contralateral/ antagonist teeth on days 7, 30, 90, and 180 after implant installation. Microbiological analysis was performed using the Checkerboard DNA-DNA hybridization technique for detection of classical oral taxa and non-oral microorganisms. Significant differences were estimated by Mann-Whitney and Friedman tests, while associations between implants/teeth and target species levels were assessed by linear regression analysis (LRA). Significance level was set at 5%.
Levels of some species were significantly higher in teeth compared to implants, respectively, at day 7 ( V.parvula , 6 × 10 5 vs 3 × 105 ; Milleri streptococci , 2 × 10 6 vs 6 × 10 5 ; Capnocytophaga spp., 2 × 10 6 vs 9 × 10 5 ; E.corrodens , 2 × 10 6 vs 5 × 10 5 ; N. mucosa , 2 × 10 6 vs 5 × 10 5 ; S.noxia , 2 × 10 6 vs 3 × 10 5 ; T.socranskii , 2 × 10 6 vs 5 × 10 5 ; H.alvei , 4 × 10 5 vs 2 × 10 5 ; and Neisseria spp., 6 × 10 5 vs 4 × 10 4 ), day 30 ( V.parvula , 5 × 10 5 vs 10 5 ; Capnocytophaga spp., 1.3 × 10 6 vs 6.8 × 10 4 ; F.periodonticum , 2 × 10 6 vs 10 6 ; S.noxia , 6 × 10 5 vs 2 × 10 5 ; H.alvei , 8 × 10 5 vs 9 × 10 4 ; and Neisseria spp., 2 × 10 5 vs 10 6 ), day 120 ( V.parvula , 8 × 10 5 vs 3 × 10 5 ; S.noxia , 2 × 10 6 vs 0; and T.socranskii , 3 × 10 5 vs 8 × 10 4 ), and day 180 ( S.enterica subsp. enterica serovar Typhi, 8 × 10 6 vs 2 × 10 6 ) (p<0.05). Implants showed significant increases over time in the levels of F.nucleatum , Gemella spp., H.pylori , P.micra , S.aureus , S.liquefaciens , and T.forsythia (p<0.05). LRA found that dental implants were negatively correlated with high levels of S. noxia and V. parvula (β=-0.5 to -0.3; p<0.05).
Early submucosal microbiota is diverse and only a few species differ between teeth and implants in the same individual. Only 7 days after implant installation, a rich microbiota can be found in the peri-implant site. After six months of evaluation, teeth and implants show similar prevalence and levels of the target species, including known and new periodontopathic species.
评估牙周健康个体中刚植入的牙种植体周围龈下微生物群在六个月内的定植动态，与相应的天然牙进行比较。
17 名接受单颗牙种植体的健康个体参与了本研究。在种植体安装后第 7、30、90 和 180 天，从所有种植体部位和对侧/对抗牙位采集龈下生物膜样本。使用 checkerboard DNA-DNA 杂交技术对经典口腔分类群和非口腔微生物进行微生物学分析。采用曼-惠特尼和弗里德曼检验估计显著性差异，同时采用线性回归分析（LRA）评估种植体/牙与目标种水平之间的相关性。显著性水平设定为 5%。
与种植体相比，一些物种在第 7 天（小韦荣球菌，6×10 5 比 3×10 5 ；米勒链球菌，2×10 6 比 6×10 5 ；二氧化碳噬纤维菌属，2×10 6 比 9×10 5 ；伴放线放线杆菌，2×10 6 比 5×10 5 ；粘膜炎莫拉菌，2×10 6 比 5×10 5 ；中间普雷沃菌，2×10 6 比 5×10 5 ；咽峡炎链球菌，2×10 6 比 3×10 5 ；迟钝真杆菌，2×10 6 比 5×10 5 ；和奈瑟菌属，2×10 6 比 4×10 4 ）、第 30 天（小韦荣球菌，5×10 5 比 10 5 ；二氧化碳噬纤维菌属，1.3×10 6 比 6.8×10 4 ；福赛斯坦纳菌，2×10 6 比 10 6 ；咽峡炎链球菌，6×10 5 比 2×10 5 ；伴放线放线杆菌，8×10 5 比 9×10 4 ；和奈瑟菌属，2×10 5 比 10 6 ）、第 120 天（小韦荣球菌，8×10 5 比 3×10 5 ；咽峡炎链球菌，2×10 6 比 0；和迟钝真杆菌，3×10 5 比 8×10 4 ）和第 180 天（伤寒沙门氏菌血清型 Typhi，8×10 6 比 2×10 6 ）（p<0.05）时，一些物种的水平显著更高。种植体随时间呈现出显著的 F.nucleatum 、消化链球菌属、幽门螺杆菌、微小消化链球菌、金黄色葡萄球菌、液化沙雷菌和福赛斯坦纳菌水平升高（p<0.05）。LRA 发现，牙种植体与高水平的咽峡炎链球菌和小韦荣球菌呈负相关（β=-0.5 至-0.3；p<0.05）。
早期黏膜下微生物群是多样的，在同一个体中，牙齿和种植体之间只有少数物种不同。在种植体安装后仅 7 天，就可以在种植体周围部位发现丰富的微生物群。经过六个月的评估，牙齿和种植体显示出相似的目标种的流行率和水平，包括已知和新的牙周病物种。
