Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal; CIMAR/CIIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto, Portugal.
Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal.
Syst Appl Microbiol. 2021 May;44(3):126189. doi: 10.1016/j.syapm.2021.126189. Epub 2021 Mar 15.
A bacterial strain, designated FF15, was isolated from the thallus surface of the macroalga Fucus spiralis sampled on a rocky beach in Porto, Portugal. Based on the 16S rRNA gene sequence, strain FF15 was affiliated to the phylum Planctomycetes. This strain forms white colonies on modified M13 medium and the cells are pear-shaped, can form rosettes, divide by polar budding and are motile. The novel isolate is mesophilic and neutrophilic with an optimum growth temperature of about 30 °C and an optimum pH for growth between 6.5 and 7.5. It showed growth over a broad range of salinities (0-9% NaCl - optimum at 1.5%). No additional vitamins are required for growth. It is cytochrome c oxidase and catalase positive. The major respiratory quinone was menaquinone 6 (MK-6). Genome sequencing revealed a genome size of 6.37 Mbp and a DNA G + C content of 54.2%. Analysis of phylogenetic markers, including similarities of the 16S rRNA gene sequence, rpoB gene sequence, as well as Percentage of Conserved Proteins (POCP), Average Nucleotide Identity (ANI) and Average Amino Acid Identity (AAI), suggest the affiliation of strain FF15 to "Bremerella", a recently described genus in the family Pirellulaceae. Based on the genotypic, phylogenetic, chemotaxonomic, physiological and biochemical characterization, we described a new species represented by strain FF15 (=CECT 8078 = LMG 31936), for which we propose the name Bremerella alba snov.
一株细菌菌株，被命名为 FF15，从葡萄牙波尔图的岩石海滩上采集的大型海藻螺旋藻的藻体表面分离出来。根据 16S rRNA 基因序列，FF15 菌株属于浮霉状菌门。该菌株在改良的 M13 培养基上形成白色菌落，细胞呈梨形，可以形成轮生体，通过极生芽殖分裂，并且具有运动性。新分离株是中温嗜中性的，最佳生长温度约为 30°C，最佳生长 pH 值在 6.5 到 7.5 之间。它在广泛的盐度范围内（0-9%NaCl-最佳盐度为 1.5%）都能生长。生长不需要额外的维生素。它是细胞色素 c 氧化酶和过氧化氢酶阳性的。主要的呼吸醌是甲萘醌 6（MK-6）。基因组测序显示基因组大小为 6.37 Mbp，DNA G+C 含量为 54.2%。对包括 16S rRNA 基因序列、rpoB 基因序列、保守蛋白百分比（POCP）、平均核苷酸同一性（ANI）和平均氨基酸同一性（AAI）在内的系统发育标记物的分析表明，FF15 菌株与“Bremerella”密切相关，“Bremerella”是最近在 Pirellulaceae 科中描述的一个新属。基于基因型、系统发育、化学分类、生理和生化特性的研究，我们描述了一株新的代表菌株 FF15（=CECT 8078= LMG 31936），我们提议将其命名为 Bremerella alba snov。
