National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China.
National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China.
Int J Mol Sci. 2023 Sep 7;24(18):13784. doi: 10.3390/ijms241813784.
, a new probiotic in recent years, can produce butyric acid and short-chain fatty acids. It has the characteristics of strong acid and alkali resistance, high temperature resistance, and strong resistance to most antibiotics, and has more advantages than other probiotics. However, the action mechanism of on is still unclear and needs further study. In this study, when was added to the basic diet, the number of living bacteria was 0, 1 × 10 and 1 × 10 CFU/g, respectively. The were randomly divided into three groups: (blank control group, experimental group 1 (1 × 10 CFU/g) and experimental group 2 (1 × 10 CFU/g)). They were fed an experimental diet for 28 days. The effects of on were studied by detecting the differences in non-specific immune indexes, intestinal microflora, and metabolites between serum and hepatopancreas. The results showed that could improve the antioxidant ability of serum and hepatopancreas, protect intestinal tissues, and promote the absorption of nutrients. At the same time, it can enhance the microbial diversity and richness of the gut flora. LC-MS metabolomics was used to detect the metabolism of intestinal flora. It was found that could up-regulate lysophosphatidylcholine in the intestine. Through the KEGG enrichment pathway, it was found that significantly different metabolites were mainly concentrated in six metabolic pathways. The purine metabolism and alanine, aspartate, and glutamate metabolism pathways showed a downward trend, indicating that the addition of to feed could reduce purine metabolism, promote the water-salt balance of the organism's cells, and reduce inflammation. In this study, it was found that the addition of certain concentrations of to feed could improve the antioxidant ability of , improve the intestinal flora environment, and promote the growth of beneficial bacteria in the gut. This can promote the body's metabolism, which is more conducive to its growth.
近年来，一种新型益生菌 能够产生丁酸和短链脂肪酸。它具有耐酸碱性强、耐高温、耐大多数抗生素的特点，比其他益生菌更具优势。然而， 对 的作用机制尚不清楚，需要进一步研究。在本研究中，当在基础日粮中添加 时，活菌数分别为 0、1×10 和 1×10 CFU/g， 将 随机分为三组：（空白对照组、实验组 1（1×10 CFU/g）和实验组 2（1×10 CFU/g））。它们被喂食实验饮食 28 天。通过检测血清和肝胰腺中非特异性免疫指标、肠道微生物区系和代谢物的差异，研究了 对 的影响。结果表明， 能提高 血清和肝胰腺的抗氧化能力，保护肠道组织，促进营养物质的吸收。同时，它可以增强肠道菌群的微生物多样性和丰富度。采用 LC-MS 代谢组学检测肠道菌群的代谢。发现 可以上调肠道中的溶血磷脂酰胆碱。通过 KEGG 富集途径发现，差异显著的代谢物主要集中在 6 条代谢途径中。嘌呤代谢和丙氨酸、天冬氨酸和谷氨酸代谢途径呈下降趋势，表明添加 到饲料中可以降低嘌呤代谢，促进机体细胞的水盐平衡，减少炎症。本研究发现，在饲料中添加一定浓度的 可以提高 的抗氧化能力，改善肠道菌群环境，促进肠道有益菌的生长。这可以促进机体的新陈代谢，更有利于其生长。
