Department of Fixed Prosthodontics, Kanagawa Dental University, Yokosuka 238-8580, Japan.
Institute of Medical Sciences, Tokyo Medical University, Shinjuku 160-8402, Japan.
Int J Mol Sci. 2023 Sep 11;24(18):13959. doi: 10.3390/ijms241813959.
Symptoms of oral discomfort such as dry mouth are common in older people wearing dentures. Such symptoms are mainly treated symptomatically. Many of these symptoms are related to saliva, and associations with salivary volume have been reported. Although denture treatment improves symptoms by increasing the amount of saliva, the effects on salivary components remain unclear. This study aimed to investigate the effects of denture treatment on salivary metabolite changes based on salivary metabolome analyses. We enrolled 21 patients requiring denture treatment. At the first visit, and after completion of denture treatment, saliva outflow was measured under resting and stimulated conditions, samples for salivary metabolite analysis were collected, and masticatory efficiency was tested. In all participants, masticatory efficiency increased after denture treatment. Moreover, the amounts of resting and stimulated saliva were increased. Using salivary metabolome analysis, 61 salivary metabolites were detected. Substantial concentration changes were observed for 4 and 21 metabolites in resting and stimulated saliva, respectively. The four metabolites common to both saliva tests had significantly lower concentrations after treatment. These results suggest that the improvement in masticatory function by dentures is related not only to salivary secretion volume, but also to salivary metabolite composition.
佩戴义齿的老年人常出现口干等口腔不适症状。这些症状主要采用对症治疗。许多症状与唾液有关，且有研究报道了唾液量与这些症状的相关性。义齿治疗通过增加唾液量来改善症状，但对唾液成分的影响尚不清楚。本研究旨在通过唾液代谢组学分析，调查义齿治疗对唾液代谢物变化的影响。我们纳入了 21 名需要义齿治疗的患者。在初次就诊时和义齿治疗完成后，分别在休息和刺激状态下测量唾液流出量，采集唾液代谢物分析样本，并检测咀嚼效率。所有参与者的咀嚼效率在义齿治疗后均提高，且休息和刺激状态下的唾液量也增加。通过唾液代谢组学分析，共检测到 61 种唾液代谢物。在休息和刺激状态下的唾液中，分别有 4 种和 21 种代谢物的浓度发生了显著变化。两种唾液测试中共同的 4 种代谢物在治疗后浓度显著降低。这些结果表明，义齿改善咀嚼功能不仅与唾液分泌量有关，还与唾液代谢物组成有关。
