Faculty of Biology, Belarusian State University, 220030 Minsk, Belarus.
Faculty of Environmental Medicine, International Sakharov Environmental Institute of Belarusian State University, 220070 Minsk, Belarus.
Int J Mol Sci. 2023 Sep 12;24(18):13987. doi: 10.3390/ijms241813987.
Dairy protein hydrolysates possess a broad spectrum of bioactivity and hypoallergenic properties, as well as pronounced bitter taste. The bitterness is reduced by complexing the proteolysis products with cyclodextrins (CDs), and it is also important to study the bioactivity of the peptides in inclusion complexes. Hydrolysates of whey and colostrum proteins with extensive hydrolysis degree and their complexes with β/γ-CD were obtained in the present study, and comprehensive comparative analysis of the experimental samples was performed. The interaction of CD with peptides was confirmed via different methods. Bioactivity of the initial hydrolysates and their complexes were evaluated. Antioxidant activity (AOA) was determined by fluorescence reduction of fluorescein in the Fenton system. Antigenic properties were studied by competitive enzyme immunoassay. Antimutagenic effect was estimated in the Ames test. According to the experimental data, a 2.17/2.78-fold and 1.45/2.14-fold increase in the AOA was found in the β/γ-CD interaction with whey and colostrum hydrolysates, respectively. A 5.6/5.3-fold decrease in the antigenicity of whey peptides in complex with β/γ-CD was detected, while the antimutagenic effect in the host-guest systems was comparable to the initial hydrolysates. Thus, bioactive CD complexes with dairy peptides were obtained. Complexes are applicable as a component of specialized foods (sports, diet).
乳蛋白水解产物具有广泛的生物活性和低过敏性，同时还具有明显的苦味。通过将蛋白水解产物与环糊精（CDs）复合，可以降低苦味，研究肽在包合物中的生物活性也很重要。本研究获得了具有广泛水解程度的乳清蛋白和初乳蛋白水解产物及其与β/γ-CD 的复合物，并对实验样品进行了综合比较分析。通过不同的方法证实了 CD 与肽的相互作用。评价了初始水解产物及其复合物的生物活性。通过 Fenton 体系中荧光素的荧光还原来测定抗氧化活性（AOA）。通过竞争性酶免疫测定法研究抗原性。在 Ames 试验中评估了抗诱变作用。根据实验数据，发现β/γ-CD 与乳清和初乳水解产物相互作用时，AOA 分别增加了 2.17/2.78 倍和 1.45/2.14 倍。检测到乳清肽与β/γ-CD 形成复合物时的抗原性降低了 5.6/5.3 倍，而在主体-客体体系中的抗诱变作用与初始水解产物相当。因此，获得了具有生物活性的乳肽 CD 复合物。这些复合物可作为特种食品（运动、饮食）的组成部分应用。
