Geng Qi, Li Huan, Wang Dan, Sheng Ruo-Cheng, Zhu He, Klosterman Steven J, Subbarao Krishna V, Chen Jie-Yin, Chen Feng-Mao, Zhang Dan-Dan
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.
Team of Crop Verticillium Wilt, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Microbiol. 2022 Feb 23;13:852571. doi: 10.3389/fmicb.2022.852571. eCollection 2022.
is a destructive soil-borne pathogen of many economically important dicots. The genetics of pathogenesis in has been extensively studied. Spt-Ada-Gcn5 acetyltransferase complex (SAGA) is an ATP-independent multifunctional chromatin remodeling complex that contributes to diverse transcriptional regulatory functions. As members of the core module in the SAGA complex in , Ada1, together with Spt7 and Spt20, play an important role in maintaining the integrity of the complex. In this study, we identified homologs of the SAGA complex in and found that deletion of the subunit () causes severe defects in the formation of conidia and microsclerotia, and in melanin biosynthesis and virulence. The effect of VdAda1 on histone acetylation in was confirmed by western blot analysis. The deletion of resulted in genome-wide alteration of the transcriptome, including genes encoding transcription factors and secreted proteins, suggesting its prominent role in the regulation of transcription and virulence. Overall, we demonstrated that VdAda1, a member of the SAGA complex, modulates multiple physiological processes by regulating global gene expression that impinge on virulence and survival in .
是许多具有重要经济价值的双子叶植物的一种破坏性土传病原体。其致病机制的遗传学已得到广泛研究。Spt - Ada - Gcn5 乙酰转移酶复合体（SAGA）是一种不依赖 ATP 的多功能染色质重塑复合体，有助于多种转录调控功能。作为 SAGA 复合体核心模块的成员，Ada1 与 Spt7 和 Spt20 一起，在维持复合体的完整性方面发挥重要作用。在本研究中，我们鉴定了 SAGA 复合体在[具体物种]中的同源物，并发现[具体亚基]亚基（VdAda1）的缺失导致分生孢子和微菌核形成、黑色素生物合成及毒力方面出现严重缺陷。通过蛋白质免疫印迹分析证实了 VdAda1 对[具体物种]组蛋白乙酰化的影响。VdAda1 的缺失导致[具体物种]转录组在全基因组范围内发生改变，包括编码转录因子和分泌蛋白的基因，表明其在转录调控和毒力方面具有重要作用。总体而言，我们证明了 SAGA 复合体的成员 VdAda1 通过调节影响[具体物种]毒力和生存的全局基因表达来调控多种生理过程。
