Meneely Julie, Greer Brett, Kolawole Oluwatobi, He Qiqi, Elliott Christopher
Institute for Global Food Security, National Measurement Laboratory: Centre of Excellence in Agriculture and Food Integrity, Queen's University Belfast, 19 Chlorine Gardens, Belfast BT9 5DL, UK.
The International Joint Research Center on Food Security (IJC-FOODSEC), 113 Thailand Science Park, Pahonyothin Road, Khong Luang 12120, Thailand.
Molecules. 2023 Sep 16;28(18):6657. doi: 10.3390/molecules28186657.
The contamination of oat crops by trichothecene mycotoxins, T-2 and HT-2 is an ongoing threat to our food safety. Within the industry, there are increasing concerns about the continued and growing presence of these mycotoxins occurring in oat crops due to climate change, farming practices and the handling of crops post-harvest. To safeguard human health, monitoring these mycotoxins in foodstuffs is paramount to ensure human exposure is limited. To achieve this, effective testing regimes must be established within the industry, consisting not only of rapid, reliable, and accurate analytical methods but also efficient sampling strategies. Four commercial rapid diagnostic kits were assessed against liquid chromatography coupled to mass spectrometry and included three lateral flow devices and one enzyme-linked immunosorbent assay. One-way ANOVA showed a -value of 0.45 indicating no significant difference between the methods assessed. Qualitative analysis revealed test kits 1, 2, 3, and 4 showed false negative/false positive rates of 1.1/2.2, 7.6/0, 2.2/0, and 6.5/0 percent, respectively. Test Kit 1, the Neogen Reveal Q+ MAX for T-2/HT-2 Kit provided the most reliable, accurate and cost-effective results. Furthermore, its ease of use and no requirement for technical skill makes it applicable for on-site testing.
燕麦作物受单端孢霉烯族毒素T - 2和HT - 2污染，这对我们的食品安全构成持续威胁。在该行业内，由于气候变化、耕作方式以及收获后作物的处理，人们越来越担心这些霉菌毒素在燕麦作物中持续存在且含量不断增加。为保障人类健康，监测食品中的这些霉菌毒素对于确保人体接触量受限至关重要。要做到这一点，行业内必须建立有效的检测制度，不仅要包括快速、可靠且准确的分析方法，还要有高效的采样策略。针对液相色谱 - 质谱联用技术评估了四种商用快速诊断试剂盒，其中包括三种侧向流动装置和一种酶联免疫吸附测定法。单因素方差分析显示p值为0.45，表明所评估的方法之间无显著差异。定性分析表明，试剂盒1、2、3和4的假阴性/假阳性率分别为1.1/2.2、7.6/0、2.2/0和6.5/0%。试剂盒1，即Neogen Reveal Q + MAX T - 2/HT - 2试剂盒，提供了最可靠、准确且经济高效的结果。此外，其使用简便且无需技术技能，适用于现场检测。
