Shokrzadeh Mohammad, Motafeghi Farzaneh Sadat, Lotfizadeh Anahita, Ghorbani Mohammad, Haddadi Kohsar Azam
Department of Toxicology-Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Student Research Committee, Faculty of Dentistry, Mazandaran University of Medical Sciences, Sari, Iran.
Int J Dent. 2023 May 31;2023:4376579. doi: 10.1155/2023/4376579. eCollection 2023.
Root canal obturation is performed by gutta-percha cones and sealer. Therefore, these materials, specially sealers, must be biocompatible. This study investigated the cytotoxicity and mineralization activity of two calcium silicate-based sealers (Endoseal MTA and Ceraseal) and an epoxy resin-based sealer (AH26).
In this experiment, the cytotoxicity of Endoseal MTA, Ceraseal, and AH26 on human gingival fibroblast cells was examined using Methyl-Thiazol-Tetrazolium assay at time intervals of 24, 48, 72, and 120 hr. The mineralization activity of sealers was evaluated by Alizarin red staining assay. Prism, ver.3, software was used to perform statistical tests. One-way analysis of variance analysis, followed by Tukey's test, was used to determine the group differences. -values < 0.05 were considered statistically significant.
Cytotoxicity of sealers decreased gradually ( < 0.0001). AH26 showed the highest level of cytotoxicity ( < 0.001). In terms of cytotoxicity, no considerable differences were observed between the two-calcium silicate-based sealers ( > 0.05). AH26 showed the lowest mineralization activity ( < 0.0001). Among the calcium silicate-based sealers, mineralization and formation of calcium nodules were more frequently observed in the Endoseal MTA group ( < 0.001).
The examined calcium silicate-based sealers had less cytotoxicity and higher mineralization activity than the resin-based sealer (AH26). There was negligible difference between the cytotoxicity of the two-calcium silicate-based, but the cell mineralization caused by Endoseal MTA was higher.
根管充填是通过牙胶尖和封闭剂来完成的。因此，这些材料，尤其是封闭剂，必须具有生物相容性。本研究调查了两种硅酸钙基封闭剂（Endoseal MTA和Ceraseal）和一种环氧树脂基封闭剂（AH26）的细胞毒性和矿化活性。
在本实验中，使用甲基噻唑四唑盐比色法在24、48、72和120小时的时间间隔内检测Endoseal MTA、Ceraseal和AH26对人牙龈成纤维细胞的细胞毒性。通过茜素红染色法评估封闭剂的矿化活性。使用Prism 3软件进行统计测试。采用单因素方差分析，随后进行Tukey检验，以确定组间差异。P值<0.05被认为具有统计学意义。
封闭剂的细胞毒性逐渐降低（P<0.0001）。AH26表现出最高水平的细胞毒性（P<0.001）。在细胞毒性方面，两种硅酸钙基封闭剂之间未观察到显著差异（P>0.05）。AH26表现出最低的矿化活性（P<0.0001）。在硅酸钙基封闭剂中，Endoseal MTA组更频繁地观察到矿化和钙结节的形成（P<0.001）。
所检测的硅酸钙基封闭剂比树脂基封闭剂（AH26）具有更低的细胞毒性和更高的矿化活性。两种硅酸钙基封闭剂的细胞毒性之间差异可忽略不计，但Endoseal MTA引起的细胞矿化更高。
